Pancreas islet separation device and method

A separation device and technology of islets, applied in the field of biomedicine, can solve problems such as inconvenient operation, high cost, and complex structure, and achieve the effect of convenient operation, low cost, and simple assembly

Inactive Publication Date: 2018-06-19
SHENZHEN YUNSHAN MEDICAL MANAGEMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the deficiencies of the prior art, the present invention proposes a device and method for separating islets. The device separates the cavity into two chambers through a filter mechanism. After the pancreas is digested in one end of the chamber, the released islets are released from the first chamber. The chamber is filtered to the second chamber through the filter mechanism, and the second opening can be unscrewed intermittently to release the islet liquid in the second chamber to avoid excessive digestion of the islets; and it solves the problem of complex structure, high cost, and The problem of inconvenient operation

Method used

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  • Pancreas islet separation device and method

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Embodiment Construction

[0032] Below in conjunction with accompanying drawing and embodiment, further elaborate the present invention.

[0033] like figure 1 As shown, a device for islet separation is used for digesting the pancreas to separate islets, including a cavity 1 and a filter mechanism 2 .

[0034] The filter mechanism 2 isolates the inner chamber of the chamber body 1 into a first chamber and a second chamber. The first chamber is provided with a first opening 111 with a cover 10 . The second chamber is provided with a second opening 112 with a cover 10 .

[0035] The pancreas is placed in the first chamber, and the digestive enzyme solution is added into the first chamber from the first opening 111 . After the first opening 111 and the second opening 112 are covered with the cover 10, the cavity 1 is sealed; it is placed under a certain temperature condition, and the device is shaken (the device can be placed with the shaker function after the cover 10 is covered). in a water bath, se...

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Abstract

The invention discloses a pancreas islet separation device and method. The device comprises a cavity and a filtration mechanism, wherein the filtration mechanism divides an inner cavity of the cavityinto a first cavity and a second cavity, the first cavity is provided with a first opening, and the second cavity is provided with a second opening. In the device, the filtration mechanism divides thecavity into two cavities, after a pancreas digests in one cavity, released pancreas islet is filtered through the filter mechanism from the first cavity to the second cavity, the second opening is unscrewed intermittently, pancreatic fluid in the second cavity is released, so that the pancreas islet is not digested excessively. The device has a simple structure, is low-cost and is convenient to operate.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a device and method for islet separation. Background technique [0002] The application of islet cell transplantation to treat diabetes, as well as the use of islet cells to carry out related basic biological and pharmaceutical research, all involve the isolation of functional islet cells from pancreatic tissue. The pancreas is composed of endocrine gland islets (also known as Langerhans cells) and exocrine gland acini. [0003] At present, the basic technical principle of islet cell isolation from pancreatic tissue is to use collagenase to digest pancreatic connective tissue, release islet cells, and separate islet cells from exocrine gland cells through subsequent purification steps [Ricordi C. et al., Isolation of the elusive pig island .Surgery.1990.107:688-694]. This principle of the method can be applied to small animals (such as rats [CN1699556A]), large animals (suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12M1/12
CPCC12N5/0676C12N2509/00
Inventor 杜文军
Owner SHENZHEN YUNSHAN MEDICAL MANAGEMENT CO LTD
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