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Preparation method of cell wax block based on non-bloody cell sample and cell wax block

A cell wax block and sample technology, applied in the biological field, can solve the problems of affecting the diagnosis result, long fixation time, cell loss, etc., and achieve the effect of improving accuracy, preventing damage, and having more cell components

Inactive Publication Date: 2018-06-12
ZHANG ZHOU HALTH VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the production of existing cell wax blocks, the fixation time is long, and the cell loss is obvious, which affects the diagnostic results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of cell wax block, it is made by following preparation method:

[0042] Centrifuge 10ml of the non-blood cell sample for the first time at 3000r / min for 5min, collect the first sediment sample and the supernatant within 1cm from the first sediment sample, and centrifuge for the second time at 3000r / min for 5min Afterwards, a second sediment sample was obtained.

[0043] Add fixative to the second precipitation sample, mix well, centrifuge for the third time under the condition of 3100r / min for 5min, and obtain the third precipitation sample, dehydrate, transparent, wax-soaked and embedding the third precipitation sample. Embedded non-blood cell samples were cut into slices with a thickness of 4um.

[0044] Wherein, the fixative solution is a neutral buffered formaldehyde solution with a concentration of 4 wt% at a temperature of 4°C.

[0045] Dehydration includes: dehydration in 95% ethanol solution at 45° C. for 120 minutes, followed by dehydration in absolute...

Embodiment 2

[0049] A kind of cell wax block, it is made by following preparation method:

[0050] After centrifuging the non-bloody cell sample for the first time at 3100r / min for 5min, collect the first sediment sample and the supernatant within 0.5cm from the first sediment sample, and centrifuge for the second time at 3000r / min for 6min After that, collect the second sediment sample and the supernatant 1 cm away from the second sediment sample.

[0051] Add fixative to the second sediment sample and the supernatant 1 cm away from the first sediment sample, mix well, and centrifuge for the third time at 3000r / min for 5 minutes to obtain the third sediment sample. Dehydration, transparency, wax immersion and embedding, cut the embedded non-blood cell samples into thin slices with a thickness of 3-5um.

[0052] Wherein, the fixative is ethanol with a volume fraction of 95%.

[0053] Dehydration includes: dehydration in 90% ethanol solution at 46°C for 110 minutes, followed by dehydratio...

Embodiment 3

[0057] A kind of cell wax block, it is made by following preparation method:

[0058] After centrifuging the non-bloody cell sample for the first time at 2900r / min for 5min, collect the first sediment sample and the supernatant within 1cm from the first sediment sample, and centrifuge for the second time at 3000r / min for 6min , collect the second pellet sample and the supernatant 1 cm away from the first pellet sample.

[0059] Add fixative to the second sediment sample and the supernatant 1 cm away from the first sediment sample, mix well, and centrifuge for the third time under the condition of 3100r / min for 5 minutes to obtain the third sediment sample. Dehydration, transparency, wax immersion and embedding, cut the embedded non-blood cell samples into thin slices with a thickness of 3-5um.

[0060] Wherein, the fixative is ethanol with a volume fraction of 95%.

[0061] Dehydration includes: dehydration in 90% ethanol solution at 45°C for 110 minutes, followed by dehydra...

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Abstract

The invention relates to a preparation method of a cell wax block based on a non-bloody cell sample and a cell wax block, and relates to the field of biotechnology. The preparation method comprises the following steps: performing first centrifugation onf a non-bloody cell sample for 5-6min at under a condition of 2,900-3,100r / min; collecting athe first precipitate sample and supernate 0-1.5cm away from the first precipitate sample; performing second centrifugationprecipitation to obtain a second precipitate sample; adding stationary liquid into the second precipitate sample, and mixing uniformly; performing third centrifugation to obtain a third precipitate sample; performing dehydration, transparentizing, wax dipping and embedding on the third precipitate sample. The preparation method provided by the invention has high preparation efficiency and is easy and convenient to operate; the prepared cell wax block has the advantages of permanent storage, many cell components, complete cellstructure and clear high-power background; the diagnosis accuracy is effectively improved.

Description

technical field [0001] The invention relates to the field of biotechnology, and in particular to a method for preparing a cell wax block based on a non-blood cell sample and the cell wax block. Background technique [0002] Exfoliative cytology examination is one of the effective methods for tumor diagnosis. The small amount of hydrothorax and ascites absorbed during the preparation of ordinary smears cannot represent the status of the entire hydrothorax and ascites. Examine most of the cells in the pleural and ascites in order to observe the morphology of most cells, and the cell wax block is easy to preserve, which greatly improves the sensitivity and specificity of cytopathological diagnosis, and makes up for the limited and insufficient production of conventional cell smears. The inadequacies of multiple stains warrant further research and prospective studies. [0003] However, in the production of the existing cell wax block, the fixation time is long, and the cell los...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/40
CPCG01N1/28G01N1/4077G01N2001/4083
Inventor 唐忠辉方志达温路生
Owner ZHANG ZHOU HALTH VOCATIONAL COLLEGE
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