Diagnosis kit for rectal carcinoma prognosis early warning and application of diagnosis kit

A colorectal cancer and kit technology, applied in the biological field, can solve problems such as difficulty in eliminating the interference of tumor heterogeneity, inability to obtain tumor diagnosis, staging, and classification standards, etc.

Inactive Publication Date: 2018-06-12
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] As mentioned before, due to the obvious heterogeneity of tumors (reflected in gene mutation, subclonal composition, biological behavior, time, space, structure, function, and even the psychological state of patients, etc.), only by traditional research on the tumor itself It is difficult to rule out the interference of tumor heterogeneity, so it is impossible to obtain diagnosis, staging, and typing standards that reflect the internal laws of tumors

Method used

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  • Diagnosis kit for rectal carcinoma prognosis early warning and application of diagnosis kit
  • Diagnosis kit for rectal carcinoma prognosis early warning and application of diagnosis kit
  • Diagnosis kit for rectal carcinoma prognosis early warning and application of diagnosis kit

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Example 1. Acquisition of immune-related genes related to the prognosis of colorectal cancer

[0049] In this example, the Spearman metastasis model was used to discover the immune-related genes AXL (sequence 1), BCL3 (sequence 2), ABR (sequence 3), MAP3K1 (sequence 4), CASP8 (sequence 5), RPS6KA1 ( Sequence 6), VASP (sequence 7), SIRT1 (sequence 8), BCL2L1 (sequence 9), AKIRIN2 (sequence 10), PIN1 (sequence 11), MEF2A (sequence 12) and PCBP2 (sequence 13), using the Spearman transfer model A bioinformatics pipeline for the discovery of immune-related genes associated with colorectal cancer prognosis figure 1 shown. Specific steps are as follows:

[0050] 1. Experimental materials

[0051] 1. Human colorectal embryonic development tissue specimens and classification

[0052] The 20 cases of human colorectal embryo development samples in the present invention were taken from the artificial abortions handled by the Maternal and Child Health Hospital of Haidian Distric...

Embodiment 2

[0097] Example 2. Application of immune-related genes in the prognosis of patients with colorectal cancer

[0098] 1. Test sample

[0099] The test samples included 10 colorectal cancer surgical resection samples with overall survival information and 2 normal adult colorectal mucosa samples. The collected clinical samples were taken from the clinical samples of Zhejiang University School of Medicine, all clinical data were complete, and the sample collection process was standardized, and no obvious RNA degradation occurred (the RNA integrity was identified by the Agilent 2100 Bioanalyzer system and the score was greater than or equal to 5 points). All the above samples obtained the informed consent of the patients, and the samples combined with genetic diseases or other diseases such as diabetes or autoimmune diseases were excluded. The postoperative survival time of 10 samples were patient 1 (7.32 years), patient 2 (7.15 years), patient 3 (6.82 years), patient 4 (7.23 years)...

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Abstract

The invention discloses a diagnosis kit for rectal carcinoma prognosis early warning and an application of the diagnosis kit. According to the diagnosis kit provided by the invention, the following 13immunity related genes, which are related to rectal carcinoma prognosis, are discovered through a Spearman transfer model: an AXL gene, a BCL3 gene, an ABR gene, an MAP3K1 gene, a CASP8 gene, an RPS6KA1 gene, a VASP gene, an SIRT1 gene, a BCL2L1 gene, an AKIRIN2 gene, a PIN1 gene, an MEF2A gene and a PCBP2 gene. Based upon experiments, it is verified that the 13 immunity related genes are relatedto the rectal carcinoma prognosis; and the 13 immunity related genes, as markers, are applicable to adjuvant prediction of postoperative survival time of patients with rectal carcinoma.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a diagnostic kit for early warning of colorectal cancer prognosis and its application. Background technique [0002] Colorectal cancer (CRC) is the third most common cancer among men worldwide (746,000 new cases, accounting for 10.0% of all tumors), and the second most common among women (614,000 new cases, 9.2% of all tumors). Even though great advances have been made in the molecular mechanisms of colorectal cancer in recent years, colorectal cancer still has a high lethality rate among all cancer types. Studies have found striking tumor heterogeneity in colorectal cancer, as well as in other cancer types. In different cellular microenvironments or different clinical treatments, the formation of cell subclones caused by genome instability is the fundamental source of tumor heterogeneity. Therefore, a model that is similar to tumors in terms of cell behavior and molec...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158
Inventor 程书钧张开泰王贵齐冯林邸雪冰安宁史小雨
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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