Method for biological remediation of iron-containing and aluminum-containing mixed contaminated soil by using nano-material

A bioremediation and nanomaterial technology is applied in the field of bioremediation of mixed contaminated soil containing iron and aluminum, which can solve the problems of being unsuitable for multiple heavy metal compound pollution, limiting the application of phytoremediation technology, and low biomass, and achieves significant heavy metal absorption and enrichment. set effect, soil degradation and productivity decline improvement, plant growth promotion effect

Pending Publication Date: 2018-06-12
四川蓉加川大环保工程咨询设计有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the key to using plants for pollution remediation is to find suitable hyperaccumulative or heavy metal-tolerant plants. However, hyperaccumulative plants usually have low biomass, slow growth, and are selective to metals, so they are not suitable for the treatment of multiple heavy metal complex pollution. The application of phytoremediation technology in the treatment of various heavy metal-contaminated soils, so the combined plant-microbe remediation of contaminated soil technology still needs to be further improved

Method used

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  • Method for biological remediation of iron-containing and aluminum-containing mixed contaminated soil by using nano-material
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  • Method for biological remediation of iron-containing and aluminum-containing mixed contaminated soil by using nano-material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] First, establish potted plants that simulate heavy metal-polluted soil, the specific method is: FeSO 4 ·7H 2 O and Al 2 (SO 4 ) 3 18H 2 O Analytical reagents are added in liquid form to ordinary nutrient soil, mixed thoroughly, so that the heavy metal content is 8000mg / kg for aluminum and 3000mg / kg for iron, and it is used after one week of storage, which is the soil for the experiment.

[0033] Then, bioremediate the above-mentioned iron- and aluminum-containing heavy metal-contaminated soil using nanomaterials. Preparatory operations before restoration: take 200g of soil for the experiment, add 200g of nano-hydroxyapatite, mix well, and use it as a mixed soil. Moses Globulus and Surface Glomus are mixed according to the volume ratio of the bacteria suspension at 1:1, which is the microbial compound agent.

[0034] The specific restoration method is: spray the above-mentioned prepared microbial compound bacterial agent around the rhizosphere of the experimental so...

Embodiment 2

[0051] First, establish potted plants that simulate heavy metal-polluted soil, the specific method is: FeSO 4 ·7H 2 O and Al 2 (SO 4 ) 3 18H 2 O Analytical pure reagents are added in liquid form to ordinary nutrient soil, mixed thoroughly, so that the heavy metal content is 16000mg / kg for aluminum and 6000mg / kg for iron, and it is used after one week of storage, which is the soil for experiment.

[0052] Then, bioremediation is carried out on the soil contaminated by heavy metals containing iron and aluminum using nanomaterials. The preparatory operation before bioremediation is as follows: take 200g of soil for the experiment, add 200g of nano-hydroxyapatite, and mix well as a mixed soil. Glomus terrestrialis and Glomus moses were prepared according to the volume ratio of the bacterial suspension at 1:1 to obtain the microbial composite agent.

[0053] The specific bioremediation method is as follows: soak the Ophiopogon japonicus plant to be moved in the microbial compo...

Embodiment 3

[0068] The method of simulating soil heavy metal pollution in the field was adopted. In Heishui County, Sichuan, a 10m piece of land for planting Chinese herbal medicines was fenced off 2 land, 50cm deep. The surrounding area is sealed with anti-seepage material and filled with local field soil. The heavy metal forms were added to FeSO 4 ·7H 2 O and Al 2 (SO 4 ) 3 18H 2 O solution, mixed thoroughly, stable for one week, the determination of heavy metal content. It has been determined that the soil contains 12680mg / kg of aluminum and 4520mg / kg of iron.

[0069] After mixing Glomus moses and Glomus terrestriale at a ratio of 1:1, the roots of the well-growing Ophiopogon japonicus were soaked in the microbial complex solution for 24 hours, and then planted in the isolated soil artificially simulated by iron and aluminum pollution. A mixed soil with a mass ratio of soil to nano-hydroxyapatite of 1:1 was added to the planting pit. A total of 100 plants were planted. The ...

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Abstract

The invention discloses a method for biological remediation of iron-containing and aluminum-containing mixed contaminated soil by using a nano-material. The method comprises the following operation steps that 1, a microbial compound microbial agent is sprayed around the rhizosphere of the iron-containing and aluminum-containing soil to be moved into the plant; 2, the plant to be moved is soaked into the microbial compound microbial agent for 23-25 hours; and 3, after any one step of the step 1 and step 2, a plant is moved into a planting pit, the iron-containing and aluminum-containing polluted soil and nano-hydroxyapatite mixed soil are added into the planting pit of the plant, after the plant grows vigorously, the overground part of the plant leaves is cut, and excessive iron and aluminum in the soil are removed. According to the technical scheme, the cydiococcus globosus and the surface sacculus are selected as the microbial compound microbial agent, the microbial compound microbialagent is sprayed on the periphery of rhizosphere soil to be moved into the plant, or is used for soaking the plants to be moved, a microbial compound microbial agent and plant symbiotic relationshipis established, and the method can protect the plant from being damaged by heavy metals and promote the growth of the plant.

Description

technical field [0001] The invention relates to the technical field of bioremediation, in particular to a method for using nanomaterials to bioremediate contaminated soil containing iron and aluminum. Background technique [0002] With the development of economy, farmland, as a natural resource for human survival, is becoming more and more overwhelmed. Environmental pollution and ecological damage not only bring serious pollution to farmland, but also endanger food safety and human health. Among them, the pollution of farmland soil by heavy metals is particularly serious, which has become an important soil environmental problem facing our country. Heavy metal-contaminated soil is highly toxic, difficult to degrade, and easy to accumulate. It can accumulate in soil and organisms, enter the human body through the food chain, and damage human health. The particularity of heavy metal-contaminated soil is that it cannot be degraded by soil microorganisms. After accumulating to a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10B09C1/00
CPCB09C1/10B09C1/105B09C2101/00
Inventor 周丽蓉崔泽周冯琳之
Owner 四川蓉加川大环保工程咨询设计有限公司
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