A kind of preparation method of aquaculture water oxygen enhancer

A technology of aquaculture water body and oxygenation agent, which is applied in the field of preparation of aquaculture water body oxygenation agent, can solve the problems of slow reduction of chemical oxygen consumption, poor oxygenation effect, short effective time, etc. Growth space, the effect of increasing dissolved oxygen

Active Publication Date: 2021-03-30
清远金沣生物药品有限公司
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Problems solved by technology

[0008] The technical problem to be solved by the present invention: Aiming at the problem that the oxygen-increasing agent cannot effectively decompose the oxygen-consuming substances at the bottom after sinking into the bottom of the water, and the efficiency of reducing the chemical oxygen consumption is too slow, resulting in poor oxygen-increasing effect and short effective time. A kind of preparation method of aquaculture water oxygen enhancer

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  • A kind of preparation method of aquaculture water oxygen enhancer

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preparation example Construction

[0029] A preparation method of an oxygenating agent for aquaculture water, comprising the steps of:

[0030] (1) Take the raw materials and add them to the enrichment medium at a mass ratio of 1:10, then add liquid paraffin with 10-12% of the mass of the enrichment medium, and culture at 28-33°C for 2-3 days. Line-inoculate into the separation medium and culture at 28-35°C for 15-18 hours, pick the colony with the largest diameter and inoculate it repeatedly into the separation medium for 2-3 times to obtain purified colonies, and take the fish and shrimp feed according to the mass ratio of 1 : 100, add sterile water, keep at 40~50°C for 1~2 days, filter, take the filtrate, inoculate the purified colonies into the filtrate at 3% inoculum size, and incubate at 35~37°C, 200 rpm for 18~24 h , repeat the culture 2~3 times, take the culture solution and spread it on the casein medium, incubate at 35~37°C for 18~24 h, pick the three single colonies with the largest diameter and inoc...

Embodiment 1

[0035] Fish and shrimp feed: purchased from Guangdong Hengxing Feed Co., Ltd., which can meet the feeding needs of fish and shrimp in the growth period.

[0036] Raw material: It comes from the bottom mud of fish and shrimp breeding ponds in Taihu Lake.

[0037] Enrichment medium: in parts by mass, take 1 part of ammonium chloride, 0.2 part of magnesium chloride, 0.5 part of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 50 parts of sodium bicarbonate, 20 parts of acetone with a mass fraction of 4%. Amino acid, adjust the pH to 7.0, and sterilize at 121°C for 20min.

[0038] Separation medium: in parts by mass, take 1 part of ammonium chloride, 0.2 parts of magnesium chloride, 2 parts of yeast extract, 0.5 parts of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 20 parts of agar, 1000 parts of water, and extinguish at 121 °C Bacteria 20min.

[0039] Feed medium: take fish and shrimp feed, add sterile water and agar at a mass ratio of 1:100:20, pH 7.0...

Embodiment 2

[0048] Fish and shrimp feed: purchased from Guangdong Hengxing Feed Co., Ltd., which can meet the feeding needs of fish and shrimp in the growth period.

[0049] Raw material: It comes from the bottom mud of fish and shrimp breeding ponds in Taihu Lake.

[0050] Enrichment medium: in parts by mass, take 1 part of ammonium chloride, 0.3 part of magnesium chloride, 0.6 part of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 55 parts of sodium bicarbonate, and 25 parts of acetone with a mass fraction of 4%. Amino acid, adjust the pH to 7.0, and sterilize at 121°C for 20min.

[0051] Separation medium: in parts by mass, take 1 part of ammonium chloride, 0.3 parts of magnesium chloride, 2 parts of yeast extract, 0.7 parts of dipotassium hydrogen phosphate, 2 parts of sodium chloride, 23 parts of agar, 1000 parts of water, and extinguish at 121 °C Bacteria 20min.

[0052] Feed medium: take fish and shrimp feed, add sterile water and agar at a mass ratio of 1:100:20, pH...

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Abstract

The invention relates to the technical field of aquaculture and in particular relates to a preparation method of a culture water body oxygenation agent. According to the preparation method, culture water body microorganisms are used for screening and bacillus is mainly adopted; when residual baits, remains, excrement, antibiotics, heavy metal type medicines and the like enter a culture water body,dissolved oxygen in water is consumed to generate pollutants including organic matters, nitrate, nitrite, ammonia nitrogen and the like, and a lot of pathogenic bacteria are bred, so that the ecological balance of culture and health of cultured animals are endangered; the bacillus is used as heterotrophic probiotics with strong stress resistance and can be used for effectively absorbing and transforming inorganic and organic pollutants in the environment; when the bacillus is used for decomposing and transforming macromolecular substances in the environment in the culture water body, nutrientelements including nitrogen, phosphorus and the like are generated, so that sufficient substances needed by the growth can be provided for beneficial photosynthetic alga in the environment; in a photosynthetic metabolism process of the alga, a lot of oxygen capable of being utilized by other organisms is generated and a micro-ecological community structure is stabilized.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a preparation method of an oxygenator for aquaculture water. Background technique [0002] With the continuous improvement of aquaculture technology and aquaculture costs, in order to pursue high yields, the amount of fish and shrimp stocked per unit water area is also increasing. However, high-density farming can easily lead to low oxygen content in aquaculture ponds, especially in areas where fish and shrimp are more concentrated at the bottom of the pond, which can cause suffocation and death of fish and shrimp, especially in hot weather. Therefore, farmers will increase oxygen in the pond within a certain period of time. [0003] The dissolved oxygen in natural water is generally in the range of 8-12mg / L, which can fully meet the oxygen demand for the normal growth of various fish. However, there are many factors that consume dissolved oxygen in water, such as the respi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
CPCC02F3/34
Inventor 谷友宝郑薇林凡
Owner 清远金沣生物药品有限公司
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