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Exiguobacterium aurantiacum and application thereof in prevention and control of tomato root-knot nematode

A tomato root-knot nematode and microbacillus technology, applied in the directions of application, nematicide, microorganism-based method, etc., can solve the problems of destroying the ecological environment of crops, high residues, soil desertification, etc., achieving good tomato growth and promoting tomato growth. , the effect of fast growth

Active Publication Date: 2018-06-01
山东新禾丰作物营养有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical nematicides have significant control effects, their selectivity is poor, which damages the ecological environment of crops; it is not easy to degrade, has high residues, and is extremely unsafe for humans and animals; it destroys the soil structure and causes soil desertification; the problem of drug resistance is becoming more and more prominent
These factors have severely restricted the use of chemical pesticides

Method used

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  • Exiguobacterium aurantiacum and application thereof in prevention and control of tomato root-knot nematode
  • Exiguobacterium aurantiacum and application thereof in prevention and control of tomato root-knot nematode
  • Exiguobacterium aurantiacum and application thereof in prevention and control of tomato root-knot nematode

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Embodiment 1

[0029] 1. Isolation and purification of E. aurantiacum 8220605

[0030] Microbacterium aurantiacum (E.aurantiacum) 8220605 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from lake mud, and separation method is:

[0031] (1) Isolation of microbacteria

[0032] Lake mud samples were collected from Nansi Lake in Jining City, Shandong Province on April 15, 2014. Weigh 1 g of lake mud into 100 mL of sterile water, place it in a shaker at 30 °C at 150 rpm for 10 min, then place it in a water bath at 60 °C for 30 min, and take 100 μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium are picked and streaked on the LB medium plate, and observed regularly Colony growth. Then use the plate streak method to purify the microbacteria strains and save them by num...

Embodiment 2

[0054] 1. Fermentation process of Microbacterium aureus strain

[0055] The formula of LB liquid medium is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.1-7.3.

[0056] Solid culture medium is made up of solid material, inorganic salt and water, and the mass ratio of described solid material and inorganic salt is 98.5: 0.5; Described solid material is rice husk by mass ratio: corn flour: soybean meal: bran=55 : 25: 12: 8; the composition and mass ratio of the inorganic salt are: potassium dihydrogen phosphate 25%, magnesium sulfate 5%, ammonium sulfate 5%, light calcium carbonate 65%. Preparation method: adding an appropriate amount of water (10-15 times the quality) to dissolve the inorganic salt to obtain an inorganic salt solution; mixing the inorganic salt solution with the solid material, and adding water to finally adjust the water content of the solid medium to 40-60%, so that the Hold the solid medium in your hand, and ...

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Abstract

The invention discloses exiguobacterium aurantiacum and application thereof in prevention and control of tomato root-knot nematode. The strain is an exiguobacterium aurantiacum strain 8220605, is obtained by being separated from the lake mud of the Nansi Lake in Jining City of Shandong province, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 26, 2017,and has a preservation number of CGMCC No. 14676. Test results prove that the exiguobacterium aurantiacum strain 8220605 biological control preparation is remarkable in prevention and control effect of the tomato root-knot nematode, and the prevention and control effect reaches 74.25%; furthermore, after the exiguobacterium aurantiacum is used, tomatoes grow well and are protected from pesticide hazard.

Description

technical field [0001] The invention belongs to the technical field of plant disease biological control. Specifically, the present invention relates to a strain of Microbacterium aureus and its application in controlling tomato root-knot nematode, and a biological control agent prepared by using the Microbacterium aureus. Background technique [0002] Root-knot nematode damage is one of the main hazards in the production process of tomato crops, which can cause more than 30% reduction in tomato production, or even failure. In recent years, the planting area of ​​vegetables in protected areas such as solar greenhouses in my country has continued to expand, and the temperature and humidity of the sun are suitable, coupled with continuous cropping all year round, resulting in the increase of root-knot nematodes year by year. So far, there have been more than 80 kinds of root-knot nematodes reported, including southern root-knot nematode (Meloidogyne incognita), Javan root-knot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P5/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 季玥秀牛赡光孙淑建张薇纪乐光
Owner 山东新禾丰作物营养有限公司
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