Primer and probe for detecting siniperca chuatsi on basis of fluorescent quantitative PCR as well as kit and method thereof

Abstract

The invention discloses a detection primer and probe for rapidly identifying siniperca chuatsi on the basis of a quantitative PCR technology, and a detection kit and detection method adopting the primer and the probe. A detection primer group comprises an upstream primer FP, a downstream primer BP and a probe; and the detection kit comprises a primer solution, a PCR MIX reaction solution, deionized water, a negative control and a positive control. The detection method comprises the following steps: extracting DNA of a sample to be detected, quickly detecting the DNA of the sample to be detected by using a fluorescent quantitative PCR technology, and judging whether the sample to be detected is siniperca chuatsi or not through a real-time amplification curve. The detection primer, the probe, the kit and the method provided by the invention have the advantages of rapidity, sensitivity, specificity, easy operation and the like, and are suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and relates to a primer, a probe, a reagent kit and a method for detecting mandarin fish based on fluorescence quantitative PCR. Background technique [0002] Siniperca chuatsi (Siniperca chuatsi), belonging to the class Osteophytes, subclass Radix, and order Perciformes, is a rare and precious freshwater fish special in my country. There are few spines in the fish meat, the meat is tender, thick, delicious, and rich in nutrition. It is a famous and high-quality variety with high market price and good economic benefits. At present, there are few reports on the identification of mandarin fish, mainly relying on the traditional morphological identification method, but practitioners need to have strong professional background knowledge, and are greatly affected by subjective judgment, especially when the fish is young, damaged or corrupt. It is difficult to identify and error-prone. The ...

AI Technical Summary

Problems solved by technology

At present, there are few reports on the identification of mandarin fish, mainly relying on the traditional morphological identification method, but practitioners need to have strong professional background knowledge, and are greatly affected by subjective judgment, especially when the fish is young, damaged or corrupt. It is difficult to identify and error-prone

The detection methods based on molecular biology mainly include microsatellite molecular markers, random primer amplification polymorphism, restriction length polymorphism, etc., but these methods have disadvantages such as time-consuming, complicated operation, poor repeatability, and large errors.

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