3D co-culture model based on tumor cell and tumor-associated fibroblast
A fibroblast, tumor-related technology, applied in 3D co-culture model, new 3D co-culture model and its construction field, can solve the problem that tumor cells are difficult to be removed by chemotherapy drugs, and achieve the effect of fast three-dimensional structure and low cost
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[0025] 1. Materials
[0026] Human pancreatic cancer cell line Capan-1:
[0027] Human primary pancreatic stellate cells: derived from pancreatic cancer specimens isolated from Zhongshan Hospital affiliated to Fudan University.
[0028] DMEM medium: purchased from Thermo Company, USA,
[0029] DMEM / F12 medium: purchased from Hyclone Company of the United States,
[0030] 10% fetal bovine serum: purchased from Gibco, USA,
[0031] Trypsin:
[0032] PBS: Phosphate buffered saline (1×), 0.0067M (PO4)
[0033] Methylcellulose: purchased from Sigma, USA,
[0034] Untreated 96-well round bottom plate: purchased from Thermo Company, USA,
[0035] GFP:
[0036] RFPs:
[0037] Streptomycin-penicillin solution: purchased from Thermo Company, USA.
[0038] 2. Method
[0039] (1) Culture of primary pancreatic stellate cells
[0040] Quickly place the pancreatic cancer tissue obtained from the hospital in a 10 cm culture dish, cut it into small pieces with high-pressure steam ster...
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