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Ganoderma active component polysaccharide peptide, polysaccharide peptide contrast and polysaccharide peptide separation and purification method

An active ingredient, Ganoderma lucidum polysaccharide technology, applied in the field of separation and purification of active ingredients of Ganoderma lucidum, can solve problems such as the inability to solve the problem of bitterness, and achieve the effects of no toxic side effects, clear active ingredients, and high safety

Active Publication Date: 2018-05-18
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than 300 master's and doctoral dissertations on the theme of Ganoderma lucidum have been collected. The above-mentioned large number of research papers and domestic and foreign patent documents have carried out a lot of research and development on the purification of Ganoderma lucidum, but it still cannot solve the bitterness contained in the complex components of the existing Ganoderma lucidum. question

Method used

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  • Ganoderma active component polysaccharide peptide, polysaccharide peptide contrast and polysaccharide peptide separation and purification method
  • Ganoderma active component polysaccharide peptide, polysaccharide peptide contrast and polysaccharide peptide separation and purification method
  • Ganoderma active component polysaccharide peptide, polysaccharide peptide contrast and polysaccharide peptide separation and purification method

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Embodiment 1

[0038] A spectral feature of Ganoderma lucidum extract stock solution. Under specific spectral conditions, Ganoderma lucidum extract has a variety of high-molecular polysaccharide peptides, and its retention time is 11.875'-15.297', and the peak molecular weight is M P 1.5×10 4 -4.8×10 5 Da (see figure 1 with figure 2 ).

[0039] A kind of active polysaccharide peptide of Ganoderma lucidum, described Ganoderma lucidum polysaccharide peptide, peak position molecular weight is in M P 1.0×10 4 Ganoderma lucidum polysaccharide peptide macromolecular compound above Da.

[0040] Duanmu Ganoderma lucidum extract under specific chromatographic conditions, retention time 8.093 minutes, Mp2.8×10 4 Da, Mn1.3×10 4 Da, Mw2.0×10 4 Da, area 94.91% (see image 3 ) is the high molecular weight polysaccharide peptide of Ganoderma lucidum.

[0041] The retention time of polysaccharide peptide in Juncao Ganoderma lucidum extract was 8.731 minutes, Mp1.0×10 4 Da Mn1.1×10 4 Da, ...

Embodiment 2

[0077] For the steps of making the Ganoderma lucidum active polysaccharide peptide product of the present invention into an oral liquid, see Figure 17 .

[0078] figure 1 HPLC chart of Ganoderma lucidum polysaccharide peptide in Ganoderma lucidum extract. Chromatographic conditions: Instrument: high performance liquid chromatography Waters 1515; 2424 evaporative light detector, injection: 10ul, column temperature: 35°C, TSK G5000PW XL with TSK G3000PW XL Column string detection. The main peak content of high molecular compounds is 18.38%, and the area is 49.23%; the main peak content of low molecular compounds is 44.79%, and the area is 50.77%, and the total area is 100%.

[0079]

[0080] figure 2 HPLC chart of Ganoderma lucidum polysaccharide peptide in Juncao Ganoderma lucidum extract. Chromatographic conditions: Instrument: high performance liquid chromatography Waters1515; 2424 evaporative light detector, sample injection: 10ul, column temperature: 35°C, TSK ...

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PUM

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Abstract

The invention discloses ganoderma active component polysaccharide peptide, a polysaccharide peptide contrast and a polysaccharide peptide separation and purification method. Ganoderma is pulverized, the pulverized ganoderma is soaked with water to obtain a ganoderma water extract, purification treatment is carried out, molecular-weight-level membrane separation is performed, a 10KD dialysis membrane is adopted to intercept a high molecular weight permeable liquid, the permeable liquid undergoes ethanol gradient separation, Bio-GL, and P-10 column chromatographic separation, purification is carried out to obtain an active component polysaccharide peptide contrast, and component identification, taste identification and content determination are carried out in a high-efficiency chromatographic condition. A molecular-weight-level membrane separation is performed to separate a bitter low molecular part. Compared with traditional ethanol extraction, the method is small in risk, high in security, free of toxic or side effect, and easy for industrial production.

Description

technical field [0001] The invention relates to a method for separating and purifying the active components of Ganoderma lucidum, in particular to a method for separating and purifying polysaccharide peptides, polysaccharide peptide reference substances and polysaccharide peptides of the active components of Ganoderma lucidum. Background technique [0002] Research by scholars at home and abroad shows that there are dozens of components in the active chemical components of Ganoderma lucidum, such as polysaccharides (peptides), triterpenes, alkaloids, and sterols. He Junjie, Chen Yan, Du Meng, etc. Experimental study on multi-index evaluation of effective components of Ganoderma lucidum extracted from microemulsion, Chinese Patent Medicine, 2013, 35(3): 479-482. At present, the industrial production process mainly uses water decoction to extract polysaccharide and polysaccharide peptide components. Such as: Li Yan, Zhao Haiyan, Lu Jianning. Research on the Extraction Technol...

Claims

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Application Information

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IPC IPC(8): C07K14/37C07K1/36C07K1/34C07K1/30
CPCC07K14/37
Inventor 林树钱林冬梅王赛贞林占熺罗虹建郭嘉铭林树光
Owner FUJIAN AGRI & FORESTRY UNIV
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