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Application of chrysanthemum morifolium extract in preparation of medicine for preventing and treating cardiac hypertrophy

A technology of myocardial hypertrophy and extract, applied in the field of application of Chrysanthemum chrysanthemum extract in the preparation of drugs for preventing and treating myocardial hypertrophy

Inactive Publication Date: 2018-05-15
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports on the application of CE in the treatment of related diseases based on myocardial hypertrophy at home and abroad.

Method used

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  • Application of chrysanthemum morifolium extract in preparation of medicine for preventing and treating cardiac hypertrophy
  • Application of chrysanthemum morifolium extract in preparation of medicine for preventing and treating cardiac hypertrophy
  • Application of chrysanthemum morifolium extract in preparation of medicine for preventing and treating cardiac hypertrophy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preliminary study on the effect of Chrysanthemum chrysanthemum extract on inhibiting isoproterenol-induced myocardial hypertrophy in mice and its mechanism

[0035] 1 Materials and methods

[0036] 1.1 Experimental animals:

[0037] Clean-grade Kunming mice, both male and female, weighing (20±2) g, provided by Zhejiang Experimental Animal Center, certificate number: SCXK (Zhejiang) 2015-0002, in the barrier environment animal laboratory of Zhejiang Experimental Animal Center Feeding and experimenting.

[0038] 1.2 Drugs and reagents:

[0039] Chrysanthemum chrysanthemum extract (CE), Zhejiang Shengshi Biotechnology Co., Ltd., light yellow powder, fragrant smell, raw material ratio 10:1, total flavonoid mass fraction 36%; isoprenaline hydrochloride (isoprenaline, Iso) injection ( Shanghai Hemu Pharmaceutical Co., Ltd., batch number 160408); lactate dehydrogenase (LDH), malondialdehyde (MDA) and total superoxide dismutase (T-SOD) test kits, protein quantitat...

Embodiment 2

[0069] Example 2: Study on the protective effect of Chrysanthemum chrysanthemum extract on myocardial hypertrophy induced by abdominal aortic constriction and its mechanism.

[0070] lMaterials and methods

[0071] 1.1 Materials

[0072] 1.1.1 Main reagents and instruments

[0073] Chrysanthemum chrysanthemum extract (CE), Zhejiang Shengshi Biotechnology Co., Ltd., light yellow powder, fragrant smell, raw material ratio 10:1, total flavonoid mass fraction 36%; valsartan, China Resources Secco Pharmaceutical Co., Ltd., Batch number: 20161008; cytoplasmic calcium ion concentration fluorescent detection kit (fura-3 / AM), Shanghai Baoman Biotechnology Co., Ltd.; goat anti-mouse atrial natriuretic peptide (atrial na triuretic peptide, ANP) and β-muscle Globulin heavy chain (β-myosin heavy chain, β-MHC) polyclonal antibody, horseradish enzyme-labeled rabbit anti-goat IgG, Wuhan Boster Biotechnology Co., Ltd.; rat sphygmomanometer, Shanghai Yuyan Scientific Instrument Co., Ltd.; flu...

Embodiment 3

[0110] Example 3: Effect of Chrysanthemum chrysanthemum extract on pressure load-induced myocardial hypertrophy and renin-angiotensin-aldosterone system in rats 1 Materials and methods

[0111] 1.1 Main reagents and instruments

[0112] Chrysanthemum chrysanthemum extract (CE, total flavonoid mass fraction 36%), Zhejiang Huzhou Shengshi Biotechnology Co., Ltd., light yellow powder; Renin (PRA) detection kit, purchased from Shanghai Lingdan Biotechnology Co., Ltd.; angiotensin (AngⅡ, aldosterone ALD) and hydroxyproline detection kits, produced by Nanjing Jiancheng Bioengineering Technology Co., Ltd.; Masson staining kits were purchased from Shanghai Lianke Biotechnology Co., Ltd.; XSP-C optical microscope, Shanghai No. 1 Optical Instrument Factory ; DU-600 ultraviolet spectrophotometer, Beckman Coulter Company of the United States; 5415D high-speed centrifuge, Eppendorf Company of Germany, echocardiography (GE Company of the United States, VIVID7 model) and 13MHz high-frequency...

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Abstract

The invention provides chrysanthemum morifolium extract, chrysanthemum morifolium extract medicinal salt, or an application of a pharmaceutical composition composed of the chrysanthemum morifolium extract or the chrysanthemum morifolium extract medicinal salt as well as a pharmaceutical adjuvant accepted by human body in preparation of medicines for preventing and treating cardiac hypertrophy or diseases caused by cardiac hypertrophy. According to the invention, isoprenaline which is a beta receptor stimulant induces mice cardiac muscles is used, and a mice cardiac hypertrophy model is copied;and rat cardiac hypertrophy is induced by coarctation of abdominal aorta, a rat cardiac hypertrophy model is copied, different dosages of the traditional Chinese medicine chrysanthemum morifolium extract can be used, through research of pharmacodynamic integral animal experiment, a positive treatment effect is achieved, and a foundation is provided for screening of new medicines.

Description

(1) Technical field [0001] The invention relates to a new application of the extract of chrysanthemum chrysanthemum, in particular to the application of the extract of chrysanthemum chrysanthemum in the preparation of medicines for preventing and treating myocardial hypertrophy. (2) Background technology [0002] Chrysanthemum Morifolium Ramat (Chrysanthemum Morifolium Ramat, CM) is an important variety of chrysanthemum in my country, and it is classified as one of the eight famous medicinal materials in Zhejiang Province "Zhe Bawei". The main place of origin of Chrysanthemum chrysanthemum is Tongxiang City, Zhejiang Province. It is also one of the first batch of authentic medicinal materials approved by the Ministry of Health. Chrysanthemum chrysanthemum is a genuine chrysanthemum recorded in the 2010 edition of "Chinese Pharmacopoeia". It contains volatile oil, steroids, Triterpenes, phenols, polysaccharides and trace elements are the main components of CM. [0003] Schol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/287A61P9/04A61P9/12A61P9/00A61P3/10A61P9/10A61P7/06A61P11/00
CPCA61K36/287A61K2236/333A61K2236/39A61K2236/51
Inventor 李乐王惠莹高丽萍程杰坤陶厚权
Owner ZHEJIANG UNIV OF TECH
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