Shaking reactor

Abstract

The invention discloses a shaking reactor, which comprises a tank body, a shaking device, a reaction bag and a control device, wherein the tank body is connected onto the shaking device; the shaking device can drive the tank body to do annular rail type movement; the reaction bag is provided with a reaction cavity; the reaction bag is arranged in the tank body and is provided with a feeding opening, an inoculation opening, a material supplementing opening, an air inlet opening and an air outlet opening which communicate with the reaction cavity; the air inlet opening is positioned in the top of the reaction bag; the control device is electrically connected with the shaking device. The shaking reactor has the advantages that the shearing force damage can be avoided during the cell culture;the amplification culture process is simple.

Description

technical field [0001] The invention relates to a shaking reactor. Background technique [0002] At present, the world's biotechnology and pharmaceutical industry is developing rapidly. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells have a very broad market application prospect. Therefore, cell bioreactors used for large-scale high-density cultivation of animal cells have become the most important equipment in the biotechnology pharmaceutical industry. It largely determines the product cost, production scale and product category of biotechnology pharmaceutical companies. As cell culture has gradually become more complex, culture containers and culture methods have also become diverse for different cells and purposes. Suspension cell culture continues to improve, and the requirements for reactors are gradually increasing, inclu...

AI Technical Summary

Problems solved by technology

First of all, during the scale-up culture process, the shear force caused by the stirring paddles will cause damage to the cells, even death

Secondly, the stirred reactor generally adopts deep ventilation method, which is easy to generate foam, and at the same time, the bursting of the bubble will cause a greater shear force

In addition, in the process of expanding the culture of the stirred reactor, due to the many parameters involved and the complex fluid mechanics, it is difficult to scale up or even fail.

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