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Basophilia mimic enzyme as well as preparation method and application thereof

An enzyme-mimicking, alkalophilic technology, applied in the field of nanotechnology and imitating enzymes, to achieve the effects of easy commercial development, high peroxidase-like catalytic activity, and easy operation

Active Publication Date: 2018-05-08
广州市仟味食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using melamine to participate in the synthesis of palladium nanoparticles solves the problem that the existing mimic enzymes cannot exert catalytic activity under extremely alkaline conditions

Method used

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  • Basophilia mimic enzyme as well as preparation method and application thereof
  • Basophilia mimic enzyme as well as preparation method and application thereof
  • Basophilia mimic enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of basophilic mimetic enzyme

[0032] 100mL melamine (10mmol / L) was pre-mixed with 1mL palladium chloride aqueous solution (50mmol / L), then 5mL sodium citrate (50mmol / L) was added, and stirred at 50°C for 2 hours to obtain basophilic melamine-protected nano-palladium (ie basophilic mimetic enzyme) in water.

[0033] The morphology of the basophilic melamine-protected nano palladium prepared in this example was detected by a Japanese transmission electron microscope (JEM-2100F). Such as figure 1 As shown, the results show that the morphology of the basophilic nano-palladium prepared in this embodiment is ultra-small nanoparticles.

Embodiment 2

[0034] Example 2 Preparation of basophilic mimetic enzyme

[0035] 100mL melamine (5mmol / L) aqueous solution and 1mL palladium chloride aqueous solution (50mmol / L) were pre-mixed, then 5mL sodium citrate (50mmol / L) was added, and stirred at 50°C for 2 hours to obtain basophilic melamine-protected nanoparticles Aqueous solution of palladium.

Embodiment 3

[0036] Example 3 Preparation of basophilic mimetic enzyme

[0037] 100mL cyanuric acid (1mmol / L) aqueous solution and 1mL palladium salt (palladium chloride) aqueous solution (10mmol / L) were pre-mixed, then 5mL sodium citrate (50mmol / L) was added, stirred at 50°C for 2 hours, An aqueous solution of nano palladium protected by basophilic melamine is obtained.

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Abstract

The invention discloses a basophilia mimic enzyme as well as a preparation method and application thereof. The preparation method comprises the following steps: pre-mixing a melamine aqueous solutionand a palladium salt aqueous solution, enabling melamine molecules to be combined with palladium ions by virtue of an electrostatic acting force, adding a reducing agent, stirring, and generating basophilia melamine protective nanopalladium, thus obtaining the basophilia mimic enzyme. The preparation method disclosed by the invention is simple in synthetic condition, low in price of raw materialsand easy in commercial development. The prepared basophilia mimic enzyme has good peroxidase activity under the extreme alkaline pH condition; and moreover, the dispersity in water is good, and the application prospect in the field such as pollutant treatment, detection under the extreme alkaline condition and the like is good.

Description

technical field [0001] The invention belongs to the field of nanotechnology and simulated enzymes, and in particular relates to an basophilic simulated enzyme and its preparation method and application. Background technique [0002] Microorganisms that can grow in extreme environments are called extremophiles. Extremophiles can produce extreme enzymes that can perform functions in extreme environments and greatly expand the application space of enzymes. They are the basis for establishing high-efficiency and low-cost biotechnology processing. The research and application of extreme enzymes will be an important way to obtain the advantages of modern biotechnology, and its application potential in environmental remediation and industrial production is great. Due to their good acid, alkali and high temperature stability, low cost, simple synthesis, and large-scale preparation, nanomaterial-like enzymes have attracted the attention and research of experts in many fields. Common...

Claims

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Application Information

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IPC IPC(8): B01J31/02B22F9/24
CPCB01J31/0244B22F9/24
Inventor 栗瑞敏邓毛程李静陈维新周爱芳王瑶
Owner 广州市仟味食品有限公司
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