Adipose tissue forming method based on in-vitro induction of vascularized pedicle

Abstract

The invention relates to an adipose tissue forming method based on in-vitro induction of a vascularized pedicle. The adipose tissue forming method comprises the following steps: taking an adipofascialtissue of the vascularized pedicle as a living support, inducing seed cells with mature adipocytes as main bodies in situ, performing in-vitro perfusion and transdifferentiation by using a muscle forming culture solution to obtain skeletal muscle cells, and constructing an axial vascularized tissue engineering skeletal muscle tissue in vitro. The adipose tissue forming method has the beneficial effects: under the premise of alleviating body damage to the maximum degree, the axial vascularized tissue engineering skeletal muscle tissue is cultured for treating muscle defects.

Description

technical field [0001] The invention belongs to the technical field of surgical medicine, and in particular relates to a method for inducing muscle formation of adipose tissue with vascular pedicles in vitro. Background technique [0002] There is significant loss of muscle tissue and its repair is often caused by a variety of natural disasters, accidental injuries, firearm injuries, etc. It is a more difficult problem in clinical practice. The current clinical treatment is mainly through autologous muscle tissue transplantation as a form and function replacement. However, this method has limited available muscle and causes damage to the donor site, resulting in insufficient muscle volume or loss of function at the donor site. Therefore, there is an urgent need to study methods that can obtain a large amount of tissue-engineered muscle tissue in a short period of time, in order to meet the needs of mass and large-scale muscle tissue defect repair. With the in-depth study ...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical defect of using simple skeletal muscle satellite cell inoculation mentioned in the background technology, although skeletal muscle can be formed, it cannot meet the clinical needs of repairing large-scale skeletal muscle defects in terms of the quantity and quality of new skeletal muscle formation, The invention provides a method for inducing muscle formation from adipose tissue with vascular pedicles in vitro. The method cultivates axial-type vascularized tissue-engineered skeletal muscle tissue under the premise of minimizing body damage to treat muscle defects.

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