Micro-scale cell electro-transfection micro-fluidic chip, electro-transfection sorter and applications thereof

Abstract

The invention relates to a micro-scale cell electro-transfection micro-fluidic chip, an electro-transfection sorter and applications thereof. The micro-scale cell electro-transfection sorter comprisesan electro-transfection unit, a display screen, an exterior box, a power supply unit, a micro control unit, and a main sensor. The electro-transfection unit comprises the chip. The display screen isused to send a command to the micro control unit and receive and display feedback information from the micro control unit and the main sensor. The micro control unit is used to receive the command sent by the display screen and control the electro-transfection unit and the power supply unit. The electro-transfection unit is used to complete cell transfection. The main sensor is used to receive feedback information from the electro-transfection unit and send the feedback information to the display screen and the micro control unit. The micro-scale cell electro-transfection micro-fluidic chip comprises an injection port, a discharge port, a negative pressure channel, a positive pressure channel, and a main channel, and a 96-well plate is arranged behind the discharge port. The provided chipcan guarantee that the transfection states in the main channel are identical during the transfection process, the transfection efficiency is guaranteed, the cell quality is guaranteed by the 96-well plate, and the subsequent cell culture becomes more convenient.

Description

technical field [0001] The invention relates to the field of IPS induced pluripotent stem cells, stem cell induced differentiation, specifically a micro-cell electroporation microfluidic chip, electroporation sorting apparatus and application thereof. Background technique [0002] Through the induction of exogenous transcriptional regulatory factors, adult cells are reprogrammed into embryonic stem cell-like pluripotent cells, which are called induced pluripotent stem cells (iPS cells), and this method is called iPS technology. The birth of iPS technology has pushed stem cell research to a new level and greatly enriched the content of stem cell research. In the past 3 years, iPS technology has developed rapidly, and has achieved success in mice, humans, macaques, rats and pigs. In particular, the research on iPS in mice and humans has been deepening, and the induction method has been continuously optimized. Various cells in animal tissues can be induced into iPS cells. Sin...

AI Technical Summary

Problems solved by technology

In the current preparation process of IPS induced pluripotent stem cells, among the transfection methods used, the transfection vectors used are mainly viruses, RNA, plasmids, etc., and the transfection methods used are mainly electroporation transfection method. The electroporation transfection method has the disadvantage of low transfection efficiency. During the transfection process, most of the cells cannot be transfected due to uneven voltage, resulting in waste, and the transfected cells and untransfected cells exist at the same time. The purification of cells Causes difficulties; in order to ensure the base number of transfected cells requires a very large number of cells (more than 1 million), the number of plasmids that need to be used is extremely large, resulting in a large amount of plasmids being wasted, which inevitably leads to increased costs, so a kind of A new IPS cell transfection culture method for cell transfection

[0004] The main problems in the prior art are as follows: 1. The transfection voltage and frequency are not uniform in the existing electroporation transfection method, which causes differences in the conditions of cell transfection, resulting in differences in the quality of transfected cells; 2. The electrotransfection voltage control of the existing transfection method is troublesome and the electrotransfer efficiency is low; 3. In order to ensure that enough transfected adult cells are finally obtained in the existing electrotransfected cells, it is necessary to use at least 1 million adult cells and consume a large amount of plasmids, and the cost is unreasonable. Avoid rising; 4. Adult cells need to be purified and separated after transfection, and clone culture. In the existing method, transfected cells and untransfected cells exist at the same time, which is difficult for cell purification and consumes a lot of manpower and material resources for later cell culture.

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