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Lighting system of plate lighting microscope with variable thickness and length of plate

A lighting system and microscope technology, applied in microscopes, optics, optical components, etc., can solve problems such as changes and inability to apply light sheet fluorescence lighting microscopes, and achieve the effects of eliminating spherical aberration, compact system structure, and reducing system aberrations

Active Publication Date: 2018-05-01
SUZHOU UNIV
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  • Application Information

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Problems solved by technology

Although the beam expansion of the laser is also realized, the total length of the system changes with the beam expansion ratio, so it cannot be applied to light-sheet fluorescence illumination microscopes

Method used

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  • Lighting system of plate lighting microscope with variable thickness and length of plate
  • Lighting system of plate lighting microscope with variable thickness and length of plate
  • Lighting system of plate lighting microscope with variable thickness and length of plate

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Embodiment 1

[0040] An illumination system for a light sheet illumination microscope with variable light sheet thickness and length, such as figure 1 As shown, sequentially arranged along the optical axis from the object side to the image side: a laser light source, an afocal zoom beam expander system, a cylindrical lens 5 , and a microscopic objective lens 6 . The wavelength of the laser light source is λ=532nm, and the beam waist radius is 0.5mm. The afocal zoom beam expander system is a four-component mechanically compensated afocal zoom beam expander system of a spherical mirror, and the four-component mechanically compensated afocal zoom beam expander system is a front fixed group 1. Zoom group 2, compensation group 3, and rear fixed group 4. The focal lengths of each component are positive, negative, negative, and positive in turn. The light emitted from the laser source passes through the front fixed group and zoom The group and compensation group are imaged at the front focus of t...

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Abstract

The invention belongs to the technical field of microimaging, in particular to a lighting path of a plate lighting microscope and provides a lighting system of a plate lighting microscope with variable thickness and length of a plate. The system successively comprises a laser light source, a four-component mechanical compensation afocal zooming beam expanding system of a full spherical mirror, a cylindrical lens and a microobjective arranged along the optical axis from the object side to the image side; light emitted by the laser light source changes in aperture of a laser beam by means of theafocal zooming beam expanding system and then passes through the cylindrical lens rotating at 90 degrees by taking the optical axis as the rotating axis to form the plate light. The plate light undergoes subduction of spherical aberration generated by the afocal zooming beam expanding system and the cylindrical lens through a spherical aberration subduction microobjective, and the light intensityof the whole plate is more uniform; the plate lighting microscope can be used for observing biological samples in a certain dimensional range under the circumstance of not replacing the plate lighting system.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to an illumination light path of a light sheet illumination microscope. Background technique [0002] Light sheet illumination microscope is a new type of optical microscope. Different from the traditional fluorescence microscope, the illumination light path of the light sheet illumination microscope and the fluorescence detection light path are perpendicular to each other. The illumination beam passes through the cylindrical lens to form a thin and uniform sheet-like beam within a certain range, while the detection optical path performs detection and imaging in a direction perpendicular to the illumination optical path, and the excitation beam is limited near the focal plane of the detection objective lens. Light sheet illumination microscopy has weak phototoxicity and fast imaging speed. It is suitable for three-dimensional imaging of living tissues and lo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/00G02B27/09
CPCG02B21/0032G02B27/0966
Inventor 曾春梅姜连马锁冬
Owner SUZHOU UNIV
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