A kind of cultivation technique of straw mushroom
A technology of straw mushrooms and craftsmanship, applied in mushroom cultivation, cultivation, plant cultivation, etc., can solve the problems of low overall pollution rate, insignificant mutual infection, slow spread, etc., achieve rich nutrients, shorten the fruiting cycle, and quickly germinate Effect
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Embodiment 1
[0036] The cultivation technique of straw mushroom comprises the steps:
[0037] S1: Straw mushroom liquid strain fermentation:
[0038] (1) Test tube inoculation: the straw mushroom strain is inoculated to the test tube slope under the condition of aseptic operation, and the inoculation amount is 10%; the straw mushroom strain in the preferred step (1) is Straw mushroom V99;
[0039] The culture medium of the test tube slant of step (1), the proportioning of each raw material is as follows: potato 20%, sucrose 2%, KH 2 PO 4 0.3%, MgSO 4 0.15%, agar 2.2%, peptone 0.4%;
[0040] (2) Activation culture: ferment and cultivate the mushroom strains inoculated in step (1) at 32°C until the hyphae are overgrown; the medium in the activation includes: 2% sucrose, 0.2% peptone, and 0.2% yeast extract , KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 0.01%;
[0041] (3) Cultivate liquid strains: take 10 pieces of 5mm × 5mm mother seed strains from the activated test tube slope, and inocul...
Embodiment 2
[0074] The formula and the clinker cultivation process of a kind of straw mushroom liquid strain in Example 1 are different in that the cultivation bag of the solid strain is adopted.
[0075] Select the formula of corn cob in Table 1: Pleurotus eryngii mushroom residue=1:1, set pH=10, and the calculation method is the same as in Table 1. The size of the cultivation bag is 32.5cm×18.5cm, each bag contains about 0.6kg, and the middle of the cultivation material is punched. What solid kind adopts is the prepared test tube kind, shares 19 test tube kinds. The liquid seed adopts the prepared shake flask liquid strain, and shares 600ml. Six different experiments were set up, each with 10 repetitions. For specific settings, see Table 2-3.
[0076] Table 2-3 Different solid-liquid inoculation and inoculation volume
[0077]
[0078] Note: Each test packs 10 bags, a total of 60 bags.
[0079] Inoculation: Inoculate according to the instructions in Table 3, operate in the inocu...
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