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Activated partial thromboplastin time detection reagent and activated partial thromboplastin time detection method

A technology of thromboplastin time and detection reagents, which is applied in the field of clinical medical detection, can solve the problems of increased cost of medical units, poor stability of reagents, and increased burden on patients, and achieve the effect of accurate detection, simple reagents, and few influencing factors

Inactive Publication Date: 2018-04-20
北京众驰伟业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The determination time of the determination reagent is long and the stability is not good
[0007] Therefore, the quality of APTT reagents has become the key to obtaining accurate results and providing clinicians with ready judgments. At the same time, due to the poor stability of reconstituted reagents and increased waste, the cost of medical units has increased and the burden on patients has increased.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Preparation of Activated Partial Thromboplastin Time Detection Reagent (APTT)

[0052] The preparation method of the activated partial thromboplastin time detection reagent comprises the following steps:

[0053] (1) Peel off the fresh rabbit head, remove surface materials such as meninges, capillaries, etc., weigh (the weight of an adult rabbit brain is 5-8g), homogenate with a mortar, add and grind the rabbit brain and physiological The volume ratio of saline is 1:4, followed by physiological saline, let stand in a water bath at 54°C for 60 minutes, centrifuge at 3000rpm for 15 minutes, and take the supernatant to obtain fresh rabbit brain infusion;

[0054] (2) By volume ratio Tris 6%, 4-aminoantipyrine 0.06%, sodium chloride 0.48%, polyethylene glycol 0.12% of average molecular weight 1500, BSA 0.4%, Triton-X 0.08%, mannitol 4 %, glycine 4%, thimerosal 0.05% and 12% fresh brain infusion, adjust the pH to 7.5 after mixing, dissolve tannic acid with 5M sod...

Embodiment 2

[0057] Example 2: Preparation of Activated Partial Thromboplastin Time Detection Reagent (APTT)

[0058] The preparation method of the activated partial thromboplastin time detection reagent comprises the following steps:

[0059] (1) Peel off the fresh rabbit head, remove surface materials such as meninges, capillaries, etc., weigh (the weight of an adult rabbit brain is 5-8g), homogenate with a mortar, add and grind the rabbit brain and physiological The volume ratio of saline is 1:2, followed by physiological saline, let stand in a water bath at 65°C for 45 minutes, centrifuge at 2000rpm for 8 minutes, and take the supernatant to obtain fresh rabbit brain infusion;

[0060] (2) Tris 2%, 4-aminoantipyrine 0.01%, sodium chloride 0.1%, polyethylene glycol 0.01% with an average molecular weight of 3000, BSA 0.1%, Triton-X 0.01%, mannitol 1 %, glycine 1%, thimerosal 0.01%, and 30% fresh brain infusion, adjust the pH to 7.2 after mixing, dissolve tannic acid with 5M sodium hydro...

Embodiment 3

[0063] Example 3: Preparation of Activated Partial Thromboplastin Time Detection Reagent (APTT)

[0064] The preparation method of the activated partial thromboplastin time detection reagent comprises the following steps:

[0065] (1) Peel off the fresh rabbit head, remove surface materials such as meninges, capillaries, etc., weigh (the weight of an adult rabbit brain is 5-8g), homogenate with a mortar, add and grind the rabbit brain and physiological The volume ratio of saline is 1:8, followed by physiological saline, let stand in a water bath at 40°C for 80 minutes, centrifuge at 4000rpm for 20 minutes, and take the supernatant to obtain fresh rabbit brain infusion;

[0066] (2) Tris 12%, 4-aminoantipyrine 0.8%, sodium chloride 1.5%, polyethylene glycol 0.8% of average molecular weight 3000, BSA 1%, Triton-X 0.8%, mannitol 12% by volume %, glycine 12%, thimerosal 0.1% and 5% fresh brain infusion, adjust the pH to 7.8 after mixing, dissolve tannic acid with 5M sodium hydrox...

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Abstract

The invention belongs to a clinical medicine detection technology, and particularly relates to an activated partial thromboplastin time detection reagent and an activated partial thromboplastin time detection method, wherein the detection reagent comprises a fresh rabbit brain impregnation liquid. According to the present invention, the method has advantages of precise detection and simple operation; and the safe, reliable and good-repeatability in vitro diagnostic reagent is provided for the endogenous blood coagulation factor deficiency and heparin therapy in clinical medicine.

Description

technical field [0001] The invention belongs to clinical medical detection technology, in particular to an activated partial thromboplastin time detection reagent and a detection method thereof. Background technique [0002] Coagulation test is of great significance to the diagnosis of various clinical diseases. In addition to the screening and diagnosis of bleeding diseases, it is also used for the examination of prethrombotic state; the experimental diagnosis of disseminated intravascular coagulation bleeding disease (DIC) Medication guidance and prognosis estimation for anticoagulants. At present, there are four coagulation tests (including prothrombin time PT, activated partial thromboplastin time APTT, thrombin time TT, and fibrinogen FIB content) commonly used clinically to detect the coagulation mechanism. [0003] Thrombosis and hemostasis not only involve basic medicine, but are also closely related to diseases in multiple clinical disciplines (including Department...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 杨军京
Owner 北京众驰伟业科技发展有限公司
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