Method for evaluating water environment disturbance based on characteristic Raman scattering of alga cells

Abstract

The invention relates to a method for evaluating the water environment disturbance based on characteristic Raman scattering of alga cells, belonging to the technical field of water environment evaluation. The method comprises the following steps: (S1) culturing alga cells under different simulated water environment disturbance factors; (S2) carrying out sampling representation on alga liquid in aculturing period, carrying out Raman imaging on the alga cells by virtue of a confocal microscope Raman spectrometer, and carrying out pretreatment on a Raman spectrum of the alga cells; (S3) analyzing characteristic Raman spectrums of the alga cells under different culturing conditions, so as to obtain the Raman difference of characteristic fingerprints of the alga cells, and determining the category of the alga cells; (S4) establishing a water environment disturbance evaluation model based on the characteristic Raman scattering of the alga cells; and (S5) evaluating and predicting a water environment according to the water environment disturbance evaluation model established in the steps (S4). According to the method, the characteristic Raman spectrum of the alga cells is established andis utilized for predicting the disturbances of different environments, so that the rapid evaluation of the water environment disturbance is realized.

Description

technical field [0001] The invention belongs to the technical field of water environment assessment, and relates to a water environment disturbance assessment method based on algae cell characteristic Raman scattering. Background technique [0002] Under normal circumstances, small disturbances in the water environment, such as water eutrophication, water light, and water turbulence, can be continuously monitored by professional instruments to monitor the nitrogen and phosphorus content and light intensity in the water, and then the degree of eutrophication of the water body can be calculated. At the same time, the researchers used fluorescence, mass spectrometry, infrared and Raman to study the physiological characteristics of algae cells caused by changes in the water environment. From the perspective of patent retrieval, patent 201310413222.1 proposed a rapid detection method for water comprehensive toxicity based on algal chlorophyll fluorescence; Change trends There ar...

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Problems solved by technology

From the perspective of patent retrieval, patent 201310413222.1 proposed a rapid detection method for water comprehensive toxicity based on algal chlorophyll fluorescence; Relatively little research has been done on the changing trend of the water environment

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