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A kind of Bacillus subtilis and its application in aquaculture

A technology of Bacillus subtilis and inoculum, applied to Bacillus subtilis and its application in aquaculture, can solve problems such as water body deterioration, and achieve the effects of removing weeds, preventing environmental pollution, and improving quality and yield

Active Publication Date: 2020-09-01
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem of deterioration of water body in the existing aquaculture mode, the object of the present invention is to provide a kind of bacillus subtilis that can effectively and rapidly purify water body, and has algae killing and prevention and treatment of aquatic pathogenic microorganisms.

Method used

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  • A kind of Bacillus subtilis and its application in aquaculture
  • A kind of Bacillus subtilis and its application in aquaculture
  • A kind of Bacillus subtilis and its application in aquaculture

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Isolation of strains

[0034] The 10-fold serial gradient dilution separation method was used to separate the microorganisms from the sediment collected from the sea cucumber culture pond in Hongdao City, Shandong Province in May 2014 on a 2216E nutrient plate, and then purified by the partition line method.

[0035] 2216E medium used: 5g of peptone, 1g of yeast extract, 0.01g of ferric phosphate, 30g of sodium chloride, 18g of agar powder, adjust the pH to 7.6, heat to dissolve, and finally set the volume to 1000mL, and sterilize at 121°C for 20min.

[0036] On the 2216E plate, the purified strains were subjected to plate antagonism tests with common aquatic animal pathogens by the filter paper method, and the strain NC108 with strong inhibitory effect on common aquatic animal pathogens was finally screened.

Embodiment 2

[0037] Identification of embodiment 2 bacterial strain NC108

[0038] 1. Morphological identification and detection of physiological and biochemical characteristics

[0039] Inoculate the isolated strains on 2216E plate, culture at 25°C for 48 hours, observe the growth of the colonies, pick the bacteria to prepare smears, perform Gram staining, observe the morphology of the bacteria and the formation of spores microscopically, and conduct morphological analysis. Identification. Physiological and biochemical characteristics were detected by conventional methods (Dong Xiuzhu, Cai Miaoying. Common bacterial system identification manual, first edition. Beijing, Science Press, 2001).

[0040] The results showed that the colony morphology of the strain was as attached figure 1 Shown: the colony is round, with irregular edges, opaque, stained white; a single cell is 0.7-0.8×2.2-2.8 microns, Gram-positive, the bacterium does not expand after spore formation, and the spores are 0.6-0...

Embodiment 3

[0067] Example 3 Water purification effect of Bacillus subtilis NC108.

[0068] Preparation of seed solution: Inoculate Bacillus subtilis NC108 into LB liquid medium, culture at 28°C, 150r / min, for about 20h (OD 600 greater than 1.0).

[0069] Preparation of fermented liquid: inoculate the above seed culture liquid in LB liquid medium and carry out fermentation and cultivation for 20h (bacterial liquid concentration ≥ 10 8 cfu / mL).

[0070] Add 1mL of fermentation broth to the 72L cultured Penaeus vannamei mariculture pond that has not changed the water for 3 days. The control group does not add any substances. After 24h and 48h after inoculation, according to the national standard "Marine Monitoring Specification Part IV: Seawater Analysis GB 17378.4- 2007" to detect nitrite nitrogen, ammonia nitrogen, sulfide, dissolved oxygen, pH and other indicators in water, and analyze the changes of each indicator. The ammonia-nitrogen detection adopts the indophenol blue spectrophoto...

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Abstract

The invention discloses a bacillus subtilis and its application in aquaculture, belonging to the technical field of aquatic microorganisms. The Bacillus subtilis is Bacillus subtilis (Bacillus subtilis) NC108, which was preserved on April 10, 2017 in: General Microbiology Center of China Committee for the Collection of Microbial Cultures, the preservation number is CGMCC NO: 14010, and the address is: Chaoyang, Beijing No. 3, Courtyard No. 1, Beichen West Road, District. The requesting unit for preservation is Qingdao Agricultural University. The Bacillus subtilis NC108 described in the present invention can rapidly reduce ammoniacal nitrogen and nitrite content in the water body, degrade algae, and has certain antagonism to Apostichopus rot skin syndrome; it can reduce the consumption of chemical pesticides and its effect on the water body. It can prevent environmental pollution, improve the quality and output of aquatic animals, and have remarkable economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of aquatic microorganisms, and in particular relates to a bacillus subtilis and its application in aquaculture. Background technique [0002] With the gradual transformation of the aquaculture model, high-density and intensive aquaculture has exacerbated the deterioration of the water body, and the pollution of the aquaculture industry to the surrounding environment and sea areas has continued to increase. Pollution in aquaculture mainly comes from over-fed bait, N, P nutrient salts and organic matter produced by the dissolution or decomposition of aquatic animal excrement and biological remains, as well as chemical or antibiotic drug residues put in during the aquaculture process; Therefore, sediment purification has become an important measure to maintain good water quality, and it is of great significance to achieve high-yield and high-quality aquaculture. Purification is divided into physical, chemical ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34A61K35/742A61P31/04C12R1/125C02F101/16
CPCA61K2035/115C02F3/34C02F2101/16C02F2101/166C02F2303/04C02F2303/20C12N1/205C12R2001/125Y02A40/70
Inventor 李雅华咸洪泉李树文
Owner QINGDAO AGRI UNIV
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