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Brewer's yeast culture and fermentation technology thereof

A technology of Saccharomyces cerevisiae and fermentation process, which is applied in the field of Saccharomyces cerevisiae culture and its fermentation process, which can solve the problems of no liquid anaerobic fermentation metabolites, insufficient solid fermentation, low yeast activity, etc., and achieve excellent palatability , improve smell, high nutritional value

Active Publication Date: 2018-04-20
北京英惠尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The general production process of yeast culture mainly includes liquid fermentation method and solid state fermentation method. Most of the current technologies only use one of the fermentation methods, but the simple single use of liquid submerged fermentation method is more obtained from the yeast cells themselves. For non-yeast metabolically active products, only solid-state fermentation is used, often with insufficient inoculum, and low yeast activity leads to insufficient solid fermentation. There are liquid-solid two-phase fermentations that combine the two, but only the liquid stage is used as yeast. During the multiplication stage of bacteria, there is no liquid anaerobic fermentation metabolites. Low-temperature anaerobic fermentation can promote the synthesis of aromatic substances such as organic acids, esters, alcohols, ketones, and terpenes.

Method used

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  • Brewer's yeast culture and fermentation technology thereof
  • Brewer's yeast culture and fermentation technology thereof
  • Brewer's yeast culture and fermentation technology thereof

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Effect test

Embodiment 1

[0049] As an embodiment of the present invention, the fermentation process of the yeast culture comprises the following steps:

[0050] (1) Shake flask seed culture (first-level and second-level seed liquid): take Saccharomyces cerevisiae strain Sa-10 preserved at ultra-low temperature (for example, liquid nitrogen at -196°C) for activation, and then inoculate the activated Saccharomyces cerevisiae 1~2 loops into the primary shake flask culture medium, shake culture at 28~30°C, 180~200rpm for 17~24 hours to obtain the first grade seed liquid, and then inoculate the first grade seed liquid into the secondary shake flask medium, 28° C. to 30° C., 180 to 200 rpm shaker shake culture for 20 to 24 hours to obtain shake flask seed culture solution.

[0051] Wherein, the primary shake flask medium and the secondary shake flask medium are YPD liquid medium, and the composition of the YPD liquid medium is: glucose 20g, tryptone 20g, yeast extract powder 10g, 1000ml water, natural pH; ...

Embodiment 2

[0064] As an embodiment of the present invention, the fermentation process of the yeast culture comprises the following steps:

[0065] (1) Shake flask seed culture (first-level and second-level seed liquid): take Saccharomyces cerevisiae strain Sa-10 preserved at ultra-low temperature (for example, liquid nitrogen at -196°C) for activation, and then inoculate the activated Saccharomyces cerevisiae 1~2 loops into the primary shake flask culture medium, shake culture at 28~30°C, 180~200rpm for 17~24 hours to obtain the first grade seed liquid, and then inoculate the first grade seed liquid into the secondary shake flask medium, 28° C. to 30° C., 180 to 200 rpm shaker shake culture for 20 to 24 hours to obtain shake flask seed culture solution.

[0066] Wherein, the primary shake flask medium and the secondary shake flask medium are YPD liquid medium, and the composition of the YPD liquid medium is: glucose 10g, tryptone 10g, yeast extract powder 5g, 1000ml water, natural pH; T...

Embodiment 3

[0079] As another embodiment of the present invention, the fermentation process of the yeast culture comprises the following steps:

[0080] (1) Shake flask seed culture (first-level and second-level seed liquid): take Saccharomyces cerevisiae strain Sa-10 preserved at ultra-low temperature (for example, liquid nitrogen at -196°C) for activation, and then inoculate the activated Saccharomyces cerevisiae 1~2 loops into the primary shake flask culture medium, shake culture at 28~30°C, 180~200rpm for 17~24 hours to obtain the first grade seed liquid, and then inoculate the first grade seed liquid into the secondary shake flask medium, 28° C. to 30° C., 180 to 200 rpm shaker shake culture for 20 to 24 hours to obtain shake flask seed culture solution.

[0081] Wherein, the primary shake flask medium and the secondary shake flask medium are YPD liquid medium, and the composition of the YPD liquid medium is: glucose 15g, tryptone 30g, yeast extract powder 15g, 1000ml water, natural ...

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Abstract

The invention discloses a brewer's yeast culture and a fermentation technology thereof. The fermentation technology of the brewer's yeast culture in the invention comprises the following steps: (1) shake flask seed culture; (2) liquid deep oxidized fermentation; (3) liquid low-temperature anaerobic fermentation; (4) solid enzymolysis aerobic fermentation; (5) solid anaerobic fermentation; and (6)solid-state high-temperature wall-breaking autolysis, and thus obtaining the brewer's yeast culture. Sufficient fermentation is carried out on brewer's yeast in the stages of liquid fermentation and solid fermentation, and more metabolites are generated, so that the product effect is improved. The brewer's yeast culture prepared through the method provided by the invention has unique aromatic odor, and after high-temperature wall breaking, probiotic ingredients such as yeast cell walls and contents can exert the effects, of food calling, irritability resistance and intestinal prebiotics, whichare not owned or embodied for viable organisms.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a culture of Saccharomyces cerevisiae and a fermentation process thereof. Background technique [0002] Saccharomyces cerevisiae culture is a kind of microecological preparation, which is formed by full fermentation of specific yeast species according to specific process and specific medium. This micro-ecological product is different from products containing active yeast cells. Its main active ingredient is the extracellular metabolites produced by Saccharomyces cerevisiae during the fermentation process. Cell contents and yeast cell walls are rich in B vitamins, minerals, small peptides, organic acids, vitamins, nucleotides, mannan oligosaccharides, β-glucans and "unknown growth-promoting factors", etc. As the nutritional substrate of probiotics in the gastrointestinal tract of animals, it can effectively stimulate the growth of probiotics, regulate the intestina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12R1/865
CPCC12N1/18
Inventor 梁廷银王宏陈鹏余际国
Owner 北京英惠尔生物技术有限公司
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