Agricultural waste utilization method
A technology for agricultural waste and agricultural waste, applied in the field of agricultural waste utilization, can solve problems affecting the development of pineapple processing enterprises, etc., and achieve the effects of eliminating bad pollution, eliminating environmental pollution and making full use of resources
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Embodiment 1
[0024] A method for utilizing agricultural waste, characterized in that it comprises the following steps:
[0025] a. Pick a ring of bacteria from the original starting strain, inoculate it into the seed medium, and culture it on a shaker at 36°C-37°C, 150-170rpm, for 16-20 hours until OD600=0.7-0.9 , stop culturing, as seed solution;
[0026] b. Inoculate the seed solution with 1%-5% inoculum in the liquid expansion LB medium (bacteria of the genus Pseudomonas stutzeri, bacteria of the genus Bacillus), PYG medium (bacteria of the genus Lactic acid bacteria), 36°C-37°C Under the temperature, 150-170rpm, shaker culture 20-28 hours. The fermentation culture liquid of various bacterial strains is mixed evenly according to the ratio of 1:1, and is used as the fermentation strain liquid of agricultural waste;
[0027] c. Inoculate the agricultural waste fermentation seed liquid in b into agricultural waste with a water content of 25%-40% with an inoculation amount of 1%-5%, mix e...
Embodiment 2
[0031] On the basis of Example 1, the bacterial strain seed culture medium in step a contains the glucose of 0.5%-1.0%, the lactose of 0.3%-0.5%, the sodium chloride of 0.3%-0.5%, the sodium chloride of 0.5%- 1.0% peptone and 0.5%-1.0% yeast extract; the LB medium described in step b contains 0.3%-0.5% sodium chloride, 0.3%-0.5% peptone and 0.5%-1.0% yeast extract; The PYG medium contains 0.5%-1.0% glucose, 0.3%-0.5% lactose, 0.3%-0.5% sodium acetate, 0.5%-1.0% peptone and 0.5%-1.0% yeast extract.
Embodiment 3
[0033] On the basis of embodiment one or embodiment two, the bacterial classification described in its step a is Pseudomonas stutzeri bacterium 1 strain, Bacillus subtilis bacterium 2 strains and Lactobacillus bacterium 1 strain.
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