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A kind of tissue culture rapid propagation method of golden catalpa

A technique for tissue culture, fast propagation and golden silk, applied in the field of plant tissue culture, can solve the problems of low input-output ratio of tissue culture of golden silk, unsatisfactory effect of rapid propagation, etc., achieves high survival rate, and is beneficial to factory production. , the effect of high effective proliferation rate

Active Publication Date: 2019-08-13
SHANDONG FOREST GERMPLASM RESOURCES CENT
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Problems solved by technology

[0006] The invention provides a high effective value-added rate and a high seedling rate tissue culture medium and rapid propagation method of Chinese catalpa. ideal technical questions

Method used

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  • A kind of tissue culture rapid propagation method of golden catalpa
  • A kind of tissue culture rapid propagation method of golden catalpa
  • A kind of tissue culture rapid propagation method of golden catalpa

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Experimental program
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Embodiment 1

[0029] 1) Obtained aseptic seedlings of Chrysanthemum catalpa

[0030] From April to June, take the grafted seedlings of Catalpa to germinate new branches in the same year, remove the leaves, petioles, tips and lignified bases, cut into 3-5cm long stems with axillary buds, soak the stems with buds in saturated clean water for 10 minutes, Brush the surface lightly with a soft brush and rinse it under running water for 1 hour; on the ultra-clean workbench, treat it with 75% alcohol for 30 seconds, rinse it twice with sterile water, then treat it with 10% sodium hypochlorite solution for 3 minutes, and rinse it with sterile water for 7 minutes. cut into 1.5cm bud stem segments and inoculate them on the primary medium, the primary medium is Qj medium, N6+6-BA 1.25mg / L+IBA 0.07mg / L+agar 6.5g / L+sucrose 30g / L, PH 5.8, inoculate 1 seed per bottle, culture conditions: light-dark alternate culture, light 14h, light intensity 3000lx, temperature 25°C, dark 10h, temperature 18°C, culture ...

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Abstract

The invention provides a catalpa tissue culture rapid propagation method, and relates to the technical field of plant tissue culture. A catalpa tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium. The primary culture medium and the proliferation culture medium are Qj culture mediums: N6+6-BA 1.25 mg / L+IBA 0.07 mg / L+ agar 6.5 g / L+ sucrose 30g / L, and the rooting culture medium selects a Qg culture medium: 1 / 2MS+6-BA0.01mg / L+IBA 0.5mg / L+ agar 6.5g / L+ sucrose 15g / L. The method catalpa tissue culture rapid propagation method using the catalpa tissue culture medium includes the following steps: 1) obtaining sterile seedlings, to be more specific, selecting new shoots, treating the lengths, cleaning, disinfecting, sterilizing, cutting, inoculating into the Qj mediums, and cultivating to obtain sterile seedlings, 2) performing proliferation culture, to be more specific, treating the lengths of the sterile seedlings, transferringthe sterile seedlings to the Qj mediums, and cultivating to obtain subculture seedlings; 3) performing rooting culture, to be more specific, treating the subculture seedlings, inoculating the subculture seedlings into the Qg culture medium, and cultivating to obtain rooted seedlings; and 4) hardening seedlings and transplanting, to be more specific, transferring the rooted seedlings to a greenhouse for hardening the seedlings, transplanting the seedlings to an aperture disk, and transplanting after cultivation. The catalpa tissue culture rapid propagation method has the beneficial effects that effective multiplication rate of rooting culture is high, seedling rate is high, and factory production can be facilitated.

Description

technical field [0001] The invention provides a method for tissue culture and rapid propagation of Chinese catalpa, which relates to the technical field of plant tissue culture. Background technique [0002] Catalpa bungei'jinsiqiu'., a tall deciduous tree belonging to the genus catalpa of the family Bignoniaceae, is an extremely important local species of high-grade wood in catalpa (Catalpa bungei). It is named after the golden thread. Due to its obvious variation characteristics and certain adaptation areas, Zhang Junpei et al. believed that Catalpa is a geographical variety of Catalpa (Zhang Junpei et al., 2008). Not only is the material tough and the pattern is beautiful, but it also has the advantages of straight trunk, strong adaptability, and fast growth. It is the preferred tree species for agricultural and forestry intercropping, afforestation and landscaping. At present, the production of golden catalpa is mainly carried out by means of root cuttings and grafting...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 韩义解孝满鲁仪增咸洋孙涛王艳刘立江仝伯强刘鹍
Owner SHANDONG FOREST GERMPLASM RESOURCES CENT
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