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Molecular markers associated with male sterile mutation sites ms-15, ms-26 and ms-47 of tomatoes and application

A technology of male sterility and mutation sites, applied in the fields of agricultural biotechnology engineering and vegetable genetics and breeding, can solve the problems of long breeding cycle and low efficiency, achieve the effect of shortening the breeding cycle and improving breeding efficiency

Active Publication Date: 2018-04-06
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, the breeding cycle is long and the efficiency is low

Method used

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  • Molecular markers associated with male sterile mutation sites ms-15, ms-26 and ms-47 of tomatoes and application
  • Molecular markers associated with male sterile mutation sites ms-15, ms-26 and ms-47 of tomatoes and application
  • Molecular markers associated with male sterile mutation sites ms-15, ms-26 and ms-47 of tomatoes and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Determination of SNPs co-segregated with tomato male sterility allelic mutation sites ms-15, ms-26 and ms-47 and InDel

[0033] plant material

[0034]Tomato male sterile mutant materials 2-193(ms-15), 2-327(ms-26) and 2-837(ms-47), seeds of wild gooseberry tomato LA1589 and common cultivated tomato Monkey Marker were all from TGRC (Tomato Genetic Resource Center). Tomato male sterile mutants 2-193(ms-15), 2-327(ms-26) and 2-837(ms-47) were mixed seeds, which were fertile plants and their selfed offspring after planting The fertile strains were named 2-193F, 2-327F and 2-837F, respectively, and the sterile strains were named 2-193S (ms-15), 2-327S (ms-26) and 2-837S (ms -47). F obtained by crossing 2-327S(ms-26) with wild gooseberry tomato LA1589 1 , F 1 F 2 generational segregation groups. The sterile plant 2-327S (ms-26) was selfed or crossed with the fertile plant 2-327F and wild gooseberry tomato LA1589, and the fruit setting was observed.

[0035]...

Embodiment 2

[0060] Example 2 Development and Verification of Molecular Markers MS26D and MS15C

[0061] Design of primers for molecular markers MS15C and MS26D

[0062] According to the sequences of the above InDels and SNPs and their flanking sequences, 2 sets of primers were designed:

[0063] 1) MS15C

[0064] The forward primer MS15C-F is: 5-ACCTTTCGTTCACGAATCTCAG-3,

[0065] The reverse primer MS15C-R is: 5-GTCTGTTGATAGGCAGAGCATA-3.

[0066] 2) MS26D

[0067] The forward primer MS26D-F is: 5-TAGAAGAACCCATTGCACTTTGT-3,

[0068] The reverse primer MS26D-R1 is: 5-CAGAACCTCTCCAACATCATAATC-3,

[0069] The reverse primer MS26D-R2 is: 5-TAATTACCGCTAATGTAACGACC-3.

[0070] Polymorphism Detection of Molecular Markers MS15C and MS26D

[0071] PCR amplification

[0072] The DNA templates for MS15C and MS26D molecular marker polymorphism detection and population verification are: 2-193 (ms-15) male sterile plants and normal plants, 2-327 (ms-26) male sterile plants and normal plants, 2 ...

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Abstract

The invention belongs to the fields of agricultural biotechnology engineering and genetic breeding of vegetables, and particularly relates to molecular markers associated with male sterile mutation sites ms-15, ms-26 and ms-47 of tomatoes, a specific primer and application. The molecular markers comprise the co-dominance molecular marker MS15C used for detecting the SNP in the site ms-15, and theco-dominance molecular marker MS26D used for detecting InDels in the sites ms-26 and ms-47. The molecular markers can be employed to replace the traditional method that by selecting male sterile plants in the flowering stage, it is ensured that a tomato breeding material contains the male sterile mutation sites ms-15 or ms-26 or ms-47. The molecular markers can assist in selecting homozygous plants and hybrid plants containing the mutation sites ms-15 or ms-26 or ms-47 in the seedling stage, so that the breeding period is shortened, and the breeding efficiency is improved.

Description

technical field [0001] The invention relates to the fields of agricultural biotechnology engineering and vegetable genetic breeding, in particular to molecular markers, primers and applications related to tomato male sterility mutation sites ms-15, ms-26 and ms-47. Background technique [0002] Using the tomato male sterile line for seed production, the hybrid seed production can reach 3000kg / year. The tomato male sterile lines used include ms-10 (male sterile-10), ms-15, ms-26, ms-32, ms-35, ms-47, ps (positional sterility), ps-2 and the like. [0003] Tomato male sterile lines ms-15, ms-26 and ms-47 are almost 100% sterile, with exposed stigmas, can be directly pollinated without manual emasculation, and have normal fruit setting after pollination, so they have been used in tomato hybrid seed production . However, ms-15, ms-26, and ms-47 are all recessive mutations. If phenotyping is performed to ensure that the ms-15, ms-26 or ms-47 locus is contained in the breeding m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 黄泽军曹雪王孝宣高建昌国艳梅杜永臣
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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