Urine preservation reagent and application thereof

Abstract

The invention discloses a urine preservation reagent and an application thereof. The urine preservation reagent comprises a nucleic acid buffer, a metal ion chelating agent, an antiseptic, a cell fixing agent, and a formaldehyde quenching agent; a nucleic acid buffer is Tris-HCl, a working pH value is 7-8, the metal ion chelating agent is EDTA, the antiseptic is citric acid and / or sodium citrate,the cell fixing agent is selected from at least one of paraformaldehyde, diazolidinyl urea, imidazolidinyl urea, and diethyl urea; and the formaldehyde quenching agent is selected from at least one ofglycine, lysine, ethene diamine and arginine. The urine preservation reagent can effectively preserve free DNA in urine, degradation can be avoided, and the urine preservation reagent establishes a base for subsequently detecting the free DNA; fragmentation of cells in the urine is prevented, a genome DNA background is reduced, and free DNA detection result accuracy is guaranteed. The urine preservation reagent is the novel reagent for preserving a urine sample, and provides the guarantee for clinical detection of the urine free DNA.

Description

technical field [0001] The present application relates to the field of urine preservation, in particular to a urine preservation reagent and its application. Background technique [0002] Urine is excreted through a series of tissues (such as the bladder), in which there are exfoliated cells and free DNA of related tissues. Urine is a complex metabolic waste excreted by the human body, including various organic and inorganic metabolites; many pathological metabolites also include proteins, carbohydrates, etc.; some patients have extremely serious hematuria. At the same time, the complexity of urine components allows many microorganisms to multiply rapidly in it, and the characteristics of urine itself make the nucleic acids in it easily degraded. [0003] Clinically, lesion tissues and cells are mainly used to detect and monitor tumor patients. The disadvantages lie in the heterogeneity of tissues, the trauma to patients, and the difficulty of sampling. With the developmen...

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Problems solved by technology

[0004] However, the free DNA content in urine is very low, and it is easily degraded in the urine environment. At the same time, the leukocyte fragmentation in urine will release a large amount of genomic DNA

This makes, on the one hand, the content of free DNA in the extracted urine nucleic acid is extremely low, and the free DNA is degraded in large quantities and cannot reach the detection level; on the other hand, a large amount of genomic DNA background affects the accuracy of the test results

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