A Saccharomyces cerevisiae strain used for postharvest disease control of fruit and its application

A technology for postharvest fruit diseases and Saccharomyces cerevisiae, which is applied in the field of Saccharomyces cerevisiae, can solve the problems of lack of antibacterial spectrum strains, and the biocontrol effect is only verified on a few fruits, so as to achieve significant social and ecological benefits and avoid harm to human beings. Harm, the effect of good application prospects

Active Publication Date: 2021-05-28
山东凯普菲特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.
However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

Method used

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  • A Saccharomyces cerevisiae strain used for postharvest disease control of fruit and its application
  • A Saccharomyces cerevisiae strain used for postharvest disease control of fruit and its application
  • A Saccharomyces cerevisiae strain used for postharvest disease control of fruit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Biological properties of the wine Saccharomyces ellipsoideus strain BY36

[0022] 1. Morphological features

[0023] (1) YPDA medium (1% yeast extract powder, 2% peptone, 2% glucose, 1.8% agar, sterilized at 121°C for 20 minutes) was cultured at 26°C for 48h, and the colonies were round and white with smooth and round edges. The cell shape is ellipsoidal.

[0024] (2) After culturing in YPDA liquid medium for 24 hours, no mold was formed, the bacterial solution was turbid, and there was precipitation. Microscopically, the yeast cells were oval and budded.

[0025] 2. Molecular biological identification

[0026] Use the universal forward primer NL-1 (5'-GCATATCAATAAGCGGAGGAAAAG-3') and the reverse primer NL-4 (5'-GGTCCGTGTTTCAAGACGG-3') to PCR amplify the yeast 26S rDNA D1 / D2 region nucleic acid sequence, and PCR The sequencing results of the product were entered into the website www.NCBI.nlm.nih.gov for BLAST, the homologous sequences were downloaded from ...

Embodiment 2

[0028] Example 2 Inhibitory Effect of Saccharomyces cerevisiae BY36 on Apple Penicillium and Botrytis Botrytis

[0029] 1. Experimental protocol

[0030] Take out Saccharomyces cerevisiae BY36 from the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration 1×10 8 cells / mL of Saccharomyces cerevisiae BY36 suspension.

[0031]Activate Penicillium expansum or Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Penicillium or Botrytis cinerea spore susp...

Embodiment 3

[0039] Example 3 Inhibitory effect of wine Saccharomyces cerevisiae BY36 on pear fruit botrytis

[0040] 1. Experimental protocol

[0041] Take out Saccharomyces cerevisiae BY36 from the -80°C refrigerator, activate it with YPDA medium (10g of yeast extract powder, 20g of peptone, 20g of glucose, 18g of agar, 1000ml of deionized water, natural pH, sterilized at 121°C for 30min), and pick a single Colony into YPD liquid medium, culture at 26°C and 200r / min for 24h, centrifuge at 4000rpm for 5min, discard the supernatant, wash the collected bacteria repeatedly with sterile water for 3 times, count on a hemocytometer to prepare the concentration It is 1×108 cell / mL suspension of Saccharomyces cerevisiae BY36.

[0042] Activate Botrytis cinerea on a PDA medium plate, culture at 26°C for 7-14 days, scrape appropriate amount of spores, and prepare a concentration of 5×10 with sterile water. 4 cells / mL of Botrytis cinerea spore suspension.

[0043] Disinfect healthy and undamaged ...

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Abstract

The invention discloses a Saccharomyces ellipsoideus strain BY36 with wide antibacterial spectrum and stable effect for preventing and controlling postharvest diseases of fruits and vegetables, and a method and application thereof. The strain number of this strain is CGMCC No.14910 in the General Microbiology Center of China Microorganism Culture Collection Management Committee. The use method of the wine saccharomyces cerevisiae: activate the strain, ferment and cultivate with YPD, centrifuge, and make up 1×10 cells with sterile water 8 Cells / mL bacterial suspension; put apples, pears, grapes, strawberries, citrus or cherry tomatoes and other fruits and vegetables into the bacterial suspension, soak for 30 seconds, take out, and air dry; put them in a fresh-keeping box and store at room temperature. The wine Saccharomyces cerevisiae strain can simultaneously control apple penicillium and gray mold, pear fruit gray mold, grape gray mold, aspergillosis, black spot, anthracnose and powdery mildew, strawberry gray mold, citrus penicillium , as well as gray mold and aspergillosis of cherry tomatoes, reducing the loss caused by postharvest diseases, and has a good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits, in particular to a wine yeast (Saccharomyces ellipsoideus) used for the biological control of post-harvest diseases of fruits. The main postharvest diseases have significant control effects. Background technique [0002] Although the deterioration of fresh fruit quality is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, the long-term use of chemical pesticides not only leads to the development of resistance of pathogenic bacteria and reduces the bactericidal effect (Prusky et al., 1985...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A01N63/32A01P3/00A23B7/155C12R1/85
CPCA23B7/155A01N63/30C12N1/16C12N1/185C12R2001/85
Inventor 王友升任向峰马国为黄津津李丽萍姚婷
Owner 山东凯普菲特生物科技有限公司
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