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A kind of intelligent antibacterial coating with good cell compatibility and preparation method thereof

An antibacterial coating and compatibility technology, which is applied in the field of intelligent antibacterial coating and its preparation, can solve the problems of uncontrollable release of antibacterial agents, bacterial drug resistance, and affecting cell growth, etc. High loading efficiency and controllable preparation process

Active Publication Date: 2020-07-24
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, contact antibacterial coatings are mainly sterilized by quaternary ammonium salts, which can cause certain toxicity to cells and affect cell growth; while the release of antibacterial agents in slow-release antibacterial coatings is uncontrollable, wasting antibacterial agents, and long-term use is easy to make bacteria drug resistance

Method used

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  • A kind of intelligent antibacterial coating with good cell compatibility and preparation method thereof
  • A kind of intelligent antibacterial coating with good cell compatibility and preparation method thereof
  • A kind of intelligent antibacterial coating with good cell compatibility and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of redox stimulus-responsive γ-polyglutamic acid (γ-PGA-PDA): Weigh 2mmol of γ-polyglutamic acid and dissolve in 35mL dimethyl sulfoxide, heat to 70℃, stir to dissolve, and cool down To 30°C; add 2mmolHOBt and 2mmolEDC to activate for 10min, then add 2mmol 2-(2-pyridyldisulfide) ethylamine hydrochloride (PDA) and react at 30°C for 24h. After the reaction is completed, the reaction solution is repeatedly precipitated in absolute ethanol, and the product can be obtained by dialysis.

[0030] (2) Preparation of dopamine-modified redox stimulus-responsive γ-polyglutamic acid (γ-PGA-PDA-DA): weigh 2mmol γ-PGA-PDA into a round bottom flask, add 35mL dimethyl sulfoxide, and increase the temperature Dissolve in half at 70°C, and cool to 30°C; add 4mmolHOBt and 2mmolEDC to activate for 10min, then add 2mmol dopamine hydrochloride (DA) and react at 30°C for 24h. After the reaction is completed, the reaction solution is repeatedly precipitated and dialyzed in absolut...

Embodiment 2

[0034] (1) Preparation of redox stimulus-responsive γ-polyglutamic acid (γ-PGA-PDA): Weigh 2mmol of γ-polyglutamic acid and dissolve in 35mL dimethyl sulfoxide, heat to 70℃, stir to dissolve, and cool down To 25°C; add 2mmolHOBt and 2mmolEDC to activate for 10min, then add 3mmol 2-(2-pyridyldisulfide) ethylamine hydrochloride (PDA) and react at 30°C for 24h. After the reaction is completed, the reaction solution is repeatedly precipitated in absolute ethanol, and the product can be obtained by dialysis.

[0035] (2) Preparation of dopamine-modified redox stimulus-responsive γ-polyglutamic acid (γ-PGA-PDA-DA): weigh 2mmol γ-PGA-PDA into a round bottom flask, add 35mL dimethyl sulfoxide, and increase the temperature Dissolve in half at 70°C, and cool to 25°C; add 4mmolHOBt and 2mmolEDC to activate for 10min, then add 3mmol dopamine hydrochloride (DA) and react at 30°C for 24h. After the reaction is completed, the reaction solution is repeatedly precipitated and dialyzed in absolut...

Embodiment 3

[0039] (1) Preparation of redox stimulus-responsive γ-polyglutamic acid (γ-PGA-PDA): Weigh 2mmol of γ-polyglutamic acid and dissolve in 35mL dimethyl sulfoxide, heat to 70℃, stir to dissolve, and cool down To 25°C; add 3mmolHOBt and 2mmolEDC to activate for 20min, then add 3mmol 2-(2-pyridyldisulfide) ethylamine hydrochloride (PDA) and react at 30°C for 20h. After the reaction is completed, the reaction solution is repeatedly precipitated in absolute ethanol, and the product can be obtained by dialysis.

[0040] (2) Preparation of dopamine-modified redox stimulus-responsive γ-polyglutamic acid (γ-PGA-PDA-DA): weigh 2mmol γ-PGA-PDA into a round bottom flask, add 35mL dimethyl sulfoxide, and increase the temperature To 70°C, halve to dissolve, and cool to 25°C;

[0041] Add 4mmolHOBt and 2mmolEDC to activate for 20min, then add 3mmol dopamine hydrochloride (DA) and react at 30°C for 24h. After the reaction is completed, the reaction solution is repeatedly precipitated and dialyzed ...

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Abstract

The invention provides an intelligent antibacterial coating with good cytocompatibility and a preparation method thereof. The prepared coating can responsively release an antibacterial agent, kill bacteria and has good cytocompatibility. The preparation method of the coating material includes: firstly utilizing 2-(pyridine-2-yldisulfanyl)ethyl-1-amine hydrochloride (PDA) and dopamine hydrochloride(DA) to modify biological macromolecule gamma-polyglutamic acid (gamma-PGA) to obtain gamma-PGA-PDA-DA; further dissolving gamma-PGA-PDA-DA and an organic antibacterial agent in an organic solvent together, and preparing colloid particles loaded with an antibacterial agent by selective solvent method; and finally taking an ethanol dispersion solution of the colloid particles loaded with the antibacterial agent as the deposition solution, and using electrophoretic deposition technology to prepare an antibacterial coating on a medical implant metal material surface. The coating not only has good cytocompatibility, but also can respond to reductive substance like dithiothreitol to release the antibacterial agent and kill bacteria around the coating. In addition, the coating also has the advantages of simple preparation method, controllable conditions, wide applicable substrate, adjustable coating thickness, high loading rate of antibacterial agent and the like.

Description

Technical field [0001] The invention relates to an intelligent antibacterial coating with good cell compatibility and a preparation method thereof, belonging to the fields of biological coatings and antibacterial coatings. [0002] technical background [0003] The clinical application of biomedical metal materials is likely to cause bacterial adhesion and proliferation, further causing bacterial infections, and a series of complications such as inflammation, pain, and fever. The preparation of antibacterial coatings on the surface of metal materials has become the main method to improve the above problems. At present, antibacterial coatings are mainly divided into two types: contact type and slow-release type. The contact type antibacterial coating mainly introduces quaternary ammonium salt into the coating, which can destroy the bacterial cell membrane, cause the loss of protein and nutrients in the bacterial cell, and cause the death of the bacteria; (LingrenWang, HaoLi, Shuai C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/14A61L31/16A61L31/02A61L31/10
CPCA61L31/022A61L31/10A61L31/14A61L31/16A61L2300/216A61L2300/406A61L2300/606A61L2400/18A61L2420/02A61L2420/06C08L77/04
Inventor 刘晓亚李杨朱叶孟龙魏玮
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