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Sowing method for promoting original land germination of paphiopedilum seeds

A technology of original soil and seeds, applied in botany equipment and methods, germination equipment, soilless cultivation, etc., can solve the problems affecting germination fungi and fungal acquisition at each growth stage, and limit the in-depth development and success of Paphiopedilum plant conservation research Low efficiency and other problems, to achieve the effect of easy recovery and observation, convenient observation of the dynamic development process of embryos, and small footprint

Active Publication Date: 2018-03-27
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conclusion, at present, the success rate of seed germination and seedling development in the native place of Paphiopedilum is low, and the effect is poor, which affects the acquisition of germination fungi and fungi at each growth stage, and limits the in-depth development of research on the conservation of Paphiopedilum species

Method used

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  • Sowing method for promoting original land germination of paphiopedilum seeds
  • Sowing method for promoting original land germination of paphiopedilum seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Artificial pollination

[0035] Robust and excellent flowering plants of P. pachyderm were selected for artificial hybridization, and the plump and mature fruit pods were picked after 150 days of pollination.

[0036] (2) Production of seed clips

[0037] Use nylon cloth with a pore size of 50 μm, cut it into a rectangle of 22×20 cm, lay the cut nylon cloth flat, and fold it in half along the outer sides of the long side to the midline to obtain a new 20×11 cm rectangle, and then along the long sides of the new rectangle 4 edge seals are heat-sealed at 0.5cm, 4.5cm, 5.5cm, and 9.5cm from the outside of both ends, and there are a total of 8 edge seals on both sides, and then there are 8 rectangular cells of 5.5×4cm, two rows up and down, each There are 4 rows, and the two rows face the opening respectively, and the opening is reserved as a sowing port. Use a transparent milk straw with a diameter of 0.3cm and a length of 15.7cm to fold into a rectangular frame of 4...

Embodiment 2

[0048] (1) Artificial pollination

[0049] Robust and excellent flowering plants of Paphiopedilum longifolia were selected for artificial hybridization, and plump and mature fruit pods were picked 180 days after pollination.

[0050] (2) Production of seed clips

[0051] Use nylon cloth with a pore size of 50 μm, cut it into a rectangle of 22×20 cm, lay the cut nylon cloth flat, and fold it in half along the outer sides of the long side to the midline to obtain a new 20×11 cm rectangle, and then along the long sides of the new rectangle 4 edge seals are heat-sealed at 0.5cm, 4.5cm, 5.5cm, and 9.5cm from the outside of both ends, and there are a total of 8 edge seals on both sides, and then there are 8 rectangular cells of 5.5×4cm, two rows up and down, each There are 4 rows, and the two rows face the openings respectively, and are reserved as sowing openings. Use a transparent milk straw with a diameter of 0.4cm and a length of 15.7cm to fold it into a rectangular frame of 4...

Embodiment 3

[0062] (1) Artificial pollination

[0063] Robust and excellent flowering plants of Paphiopedilum petiolum were selected for artificial hybridization, and the plump and mature fruit pods were picked after 210 days of pollination.

[0064] (2) Production of seed clips

[0065] Use nylon cloth with a pore size of 48 μm, cut it into a rectangle of 22×20 cm, lay the cut nylon cloth flat, and fold it in half along the outer sides of the long side to the midline to obtain a new 20×11 cm rectangle, and then along the long sides of the new rectangle 4 edge seals are heat-sealed at 0.5cm, 4.5cm, 5.5cm, and 9.5cm from the outside of both ends, and there are a total of 8 edge seals on both sides, and then there are 8 rectangular cells of 5.5×4cm, two rows up and down, each There are 4 rows, and the two rows face the opening respectively, and the opening is reserved as a sowing port. Use a transparent milk straw with a diameter of 0.3cm and a length of 15.7cm to fold into a rectangular ...

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Abstract

The invention discloses a paphiopedilum seed clamp which is made of square nylon cloth, and the square nylon cloth is provided with an upper row of seed chambers and a lower row of seed chambers whichare opened in opposite directions. The inventors also establish a corresponding sowing method for promoting original land germination of paphiopedilum seeds. The seed clamp is used for sowing paphiopedilum seeds of 150-210d, and the seed clamp is folded and then put around the root of a mother plant so as to realize native sowing of paphiopedilum. The sowing method disclosed by the invention is simple and flexible in operation, realizes high germination rate and good seedling development, is convenient for observing dynamic development processes of seed embryos, is convenient for separating mycorrhizal fungi in different stages, reduces the affects on other seed embryos caused by many times of observation or material taking, is suitable for simultaneous sowing of multiple paphiopedilum seeds, and is newly developed for conservation research of reintroduction, symbiotic seed germination and the like of endangered paphiopedilum.

Description

technical field [0001] The invention belongs to the technical field of Paphiopedilum seed germination, and in particular relates to a sowing method for promoting the original germination of Paphiopedilum seed. Background technique [0002] Paphiopedilum is one of the most popular orchids. It is a rare and precious ornamental orchid widely cultivated in the world. Due to excessive human excavation and habitat destruction, the number of wild Paphiopedilum resources has rapidly decreased, and its distribution area has gradually shrunk. Critically endangered, all species of this genus are included in Appendix I of the Convention on International Trade (CITES). [0003] Like other orchids, Paphiopedilum has a large number of small seeds, but the endosperm of the seeds is missing. Under natural conditions, Paphiopediopsis seeds can only germinate normally if they live in symbiosis with fungi, and the germination rate is very low. At present, the tissue culture of Paphiopedilum is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01G31/00
CPCA01C1/02A01G31/00
Inventor 陈宝玲王华新龚建英周千淞唐遒冥唐庆龙定建陈尔苏莉花杨舒婷林茂
Owner GUANGXI FORESTRY RES INST
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