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Agricultural composite microbial agent and preparation method of same

A technology of compound bacterial agent and bacterial liquid, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of low efficiency of bacteria, high requirements for storage conditions, short shelf life of bacteria, etc., to improve The effect of raising product quality, enhancing disease resistance, and high content of humic acid

Inactive Publication Date: 2018-03-23
潍坊市华滨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. The types of domestic microbial strains are relatively single, mostly Bacillus, which is widely used because of its convenient cultivation, cheap price and long shelf life, but the application effect is general; a single strain is rarely used as a fermented fertilizer because of its metabolites (mainly enzymes, that is, enzymes) are difficult to cope with the full fermentation of organic raw materials and mineral source raw materials (inorganic nutrition)
[0005] 2. Domestic compound microbial strain compounding technology is relatively simple, mostly based on simple multi-blending, without fully considering the interaction relationship between different microorganisms, which further leads to the low efficiency of the strain
[0006] 3. It is difficult to control factors such as antagonism between microorganisms and reproduction time in compound strains
[0007] 4. Fermentation of complex microbial strains, fertilizer formula, and process technology are not yet perfect
[0008] 5. The storage effect of strain carriers on different microorganisms needs to be further studied
[0009] 6. No complex microbial strains have been found to decompose or dissolve mineral humic acid raw materials (weathered coal, lignite, etc.) to produce water-soluble humic acid
[0010] 7. The shelf life of the strain is short, and the storage conditions are high, so it needs to be stored in a designated place and a designated cold storage

Method used

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  • Agricultural composite microbial agent and preparation method of same
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  • Agricultural composite microbial agent and preparation method of same

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preparation example Construction

[0055] The preparation method of the bacterial liquid is as follows: the bacteria are cultured in the bacterial seed culture medium at 37°C for 24 hours; then cultivated in the bacterial shaker culture medium at 37°C and the shaker speed is 180r / min for 18 hours; finally in the bacteria Fermentation culture medium, 37 ℃, shaker speed is 180r / min under the fermentation culture for 36 hours, after the fermentation culture is completed, the mother liquor is obtained, which is the bacterial bacterial liquid. The spore production rate in the bacterial bacterial liquid is more than 90%, and the number of effective viable bacteria ≥5.0×10 9 cfu / mL;

[0056] Wherein, the composition of bacterial seed culture medium and bacterial shaker culture medium is identical;

[0057] Bacteria seed culture medium is: peptone 1%, yeast extract 0.5%, sodium chloride 1%, the rest is water, pH is 7.0;

[0058] The bacterial fermentation medium is: starch 5%, peptone 1.5%, sodium chloride 0.6%, the ...

Embodiment 1

[0072] Each bacterium solution obtained above is inoculated into the comprehensive culture medium for fermentation according to the volume ratio of 35% of bacteria, 35% of yeast, 10% of lactic acid bacteria, 10% of actinomycetes and 10% of filamentous fungi, and the volume ratio of inoculation is 10%, cultivated for 7 days at 30° C. with a shaker speed of 150 r / min to obtain a liquid agricultural composite bacterial agent. The effective viable bacteria detection results of the liquid agricultural composite bacterial agent are shown in Table 1.

[0073] The components and parts by weight in the comprehensive medium are: 0.08 parts of molasses, 2 parts of honey, 1 part of ascorbic acid, 2.5 parts of ammonium sulfate, 2 parts of dipotassium hydrogen phosphate, 0.2 parts of magnesium sulfate, 1 part of yeast extract, 1000 parts of water share.

[0074] Table 1: Detection results of liquid agricultural compound bacterial agents (unit: ×10 8 cfu / mL)

[0075]

[0076] After test...

Embodiment 2

[0085] Each bacterium solution obtained above is inoculated into the comprehensive culture medium for fermentation according to the volume ratio of 25% of bacteria, 25% of yeast, 20% of lactic acid bacteria, 15% of actinomycetes and 15% of filamentous fungi, and the volume ratio of inoculation is 10%, cultivated for 10 days at 30° C. with a shaker speed of 150 r / min to obtain a liquid agricultural compound bacterial agent. The effective viable bacteria detection results of the liquid agricultural compound bacterial agent are shown in Table 4.

[0086] The components and parts by weight in the comprehensive medium are: 0.08 parts of molasses, 2 parts of honey, 1 part of ascorbic acid, 2.5 parts of ammonium sulfate, 2 parts of dipotassium hydrogen phosphate, 0.2 parts of magnesium sulfate, 1 part of yeast extract, 1000 parts of water share.

[0087]Table 4: Detection results of liquid agricultural compound bacterial agents (unit: ×10 8 cfu / mL)

[0088]

[0089] After testin...

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Abstract

The invention relates to an agricultural composite microbial agent and a preparation method of same. The method is direct at defects in the prior art and includes a step of compounding bacteria, yeast, lactic acid bacteria, actinomycetes and filamentous fungus, so that the composite microbial agent has relative stability and reliability among the microorganisms, is high in fermentation efficiencyand can be preserved at normal temperature for long time. By means of the preparation method, the agricultural composite microbial agent can be processed in liquids or solid powders conveniently, final products having acidity. The product can resist infection of foreign bacteria, is free of refrigeration, is convenient to store and has long shelf life.

Description

technical field [0001] The invention belongs to the technical field of composite bacteria, and in particular relates to an agricultural composite bacteria agent and a preparation method thereof Background technique [0002] Agricultural compound bacterial agents refer to microbial preparations made of two or more microbial strains that are not antagonistic to each other and are used in agriculture. They are mainly used as components of biological compound fertilizers or as fermentation strains for agricultural fermentation. [0003] At present, there are mainly the following defects in agricultural fungicides: [0004] 1. The types of domestic microbial strains are relatively single, mostly Bacillus, which is widely used because of its convenient cultivation, cheap price and long shelf life, but the application effect is general; a single strain is rarely used as a fermented fertilizer because of its metabolites (mainly enzymes, i.e. enzymes) are difficult to cope with the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/16C12R1/125C12R1/865C12R1/25C12R1/465C12R1/69C12R1/885C12R1/645
CPCC12N1/14C12N1/16C12N1/20
Inventor 高亮谭德星
Owner 潍坊市华滨生物科技有限公司
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