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A kind of conidia microcapsule of metarhizium anisopliae and its preparation method and application

A technology of conidia and metarhizium anisopliae, applied in the direction of microcapsule preparation, microsphere preparation, botanical equipment and methods, etc., can solve the problems of short storage period and poor stress resistance, and achieve the goal of overcoming poor stress resistance and high activity , Improve the effect of UV resistance

Active Publication Date: 2020-07-03
GUANGDONG ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of this invention is to provide a kind of conidia of Metarhizium anisopliae with long storage period and strong stress resistance for the shortcomings of short storage period and poor stress resistance in the actual application of Metarhizium anisopliae insecticides in the prior art Microcapsule and its preparation method and application

Method used

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  • A kind of conidia microcapsule of metarhizium anisopliae and its preparation method and application
  • A kind of conidia microcapsule of metarhizium anisopliae and its preparation method and application
  • A kind of conidia microcapsule of metarhizium anisopliae and its preparation method and application

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Embodiment 1

[0037] Embodiment 1: the preparation of metarhizium anisopliae conidia microcapsules

[0038] figure 1 Be the preparation method flowchart of Metarhizium anisopliae conidia microcapsules, detailed method is as follows:

[0039] S1. Capsule core preparation: Mix conidia powder of Metarhizium anisopliae and simethicone oil at a mass ratio of 1:20 (g / g), and mix thoroughly by vortex stirring at room temperature to obtain a core material solution.

[0040] S2. Emulsion preparation: mix equal volumes of gelatin A and gum arabic solutions with a concentration of 2% w / v, stir evenly, and prepare wall materials; place them at room temperature or in a water bath at 25-80°C to prevent re- Form a gel. Then add the core material solution with the same quality as the wall material, shear the mixed solution for 10 minutes at an ultra-high speed speed of 10000r / min for emulsification, and obtain an oil-in-water emulsion with uniform oil droplet size, uniform dispersion and stability.

[0...

Embodiment 2

[0042] Embodiment 2: single factor optimization microcapsule preparation process

[0043] Prepare Metarhizium anisopliae conidia microcapsule powder according to the method described in Example 1, adopt single factor optimization method for wall material ratio, wall material mass fraction, core-wall ratio, vesicle-forming pH, vesicle-forming temperature, stirring speed to microcapsule The preparation process is optimized. The results showed that under certain treatment conditions, the ratio of wall material (mass of gelatin: mass of gum arabic), the mass fraction of wall material, the ratio of core to wall and the stirring speed had a great influence on the formation of microcapsules, while the pH factor had a great influence on the formation of microcapsules. The shape has little effect ( Figure 2-5 , Figure 7 ). When the wall material ratio is 3:1 or 2:1, the microcapsules stick together and form a group, while when the wall material ratio is 1:1 and 1:2, the microcapsu...

Embodiment 3

[0044] Embodiment 3: the preparation method (A) of the metarhizium anisopliae conidia microcapsule of optimization

[0045] S1. Capsule core preparation: mix conidia powder of Metarhizium anisopliae and simethicone oil at a mass ratio of 0.5:20 (g / g), and mix thoroughly by vortex stirring at room temperature to obtain a water content of less than 5%. , spore content 1×10 11 The oil suspension of conidia of Metarhizium anisopliae more than one / mL is the core material solution.

[0046] S2. Emulsion preparation: take an appropriate amount of type A gelatin, dissolve it under the condition of heating in a water bath at 40°C to form a type A gelatin solution with a mass concentration of 2% w / v, and take another amount of gum arabic equal to that of type A gelatin, add distilled water and put in a water bath Heating to 40°C, stirring slowly at a rate of 500r / min to form a gum arabic solution with a mass concentration of 2% w / v; both the type A gelatin and the gum arabic solution w...

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Abstract

The invention discloses a green muscardine fungus conidium microcapsule and a preparation method and application thereof. The green muscardine fungus conidium microcapsule disclosed by the invention comprises a core material and a wall material and is prepared by adopting complex coacervation, wherein the core material comprises green muscardine fungus conidium powder and simethicone; the wall material comprises A-type gelatin and Arabic gum; the microcapsule is regularly shaped, and the particle size is about 35 to 50 microns. According to the microcapsule disclosed by the invention, the ultraviolet resistance of green muscardine fungus is obviously improved, the storage time of conidium is prolonged, and the insecticidal toxicity for red imported fire ants is also relatively high; the defect that the field application stress resistance of green muscardine fungus conidium is low is overcome, the control effect for agricultural and forest pests is obviously improved, and the microcapsule has important market applications and popularization values.

Description

technical field [0001] The invention belongs to the technical field of using entomogenic fungi to prevent and control forestry and agricultural pests, in particular to a conidia microcapsule of Metarhizium anisopliae and its preparation method and application. Background technique [0002] Extensive use of highly toxic chemical insecticides is still a commonly used method in the control of agricultural and forestry pests. It not only pollutes the environment and affects food safety, but also kills natural enemies and pests are prone to resistance. With the increasing social demand for pollution-free agricultural and forestry products and yearning for a beautiful ecological environment, the country is increasingly emphasizing the concept of green, coordinated and sustainable economic development, paying more attention to reducing the use of chemical pesticides, and vigorously promoting the development of biological pesticides. [0003] Entomogenic fungi are a class of fungi t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01N25/28A01N25/22A01P7/04B01J13/10
CPCA01N25/22A01N25/28A01N63/30B01J13/10
Inventor 邱华龙秦长生徐金柱赵丹阳谭晓霞揭育泽杨华练涛赖国栋黄兆光张美华
Owner GUANGDONG ACAD OF FORESTRY
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