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In-vitro culture and rapid propagation method of lithops

A kind of technology of in vitro culture, Lithops

Active Publication Date: 2018-03-20
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Based on the problems existing in the prior art, the purpose of the present invention is to provide a method for in vitro culture and rapid propagation of Lithops, which can quickly cultivate Lithops, and solve the problem of slow growth and low reproductive efficiency of Lithops, and that normal cultivation cannot meet the needs of the market. demand problem

Method used

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  • In-vitro culture and rapid propagation method of lithops
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  • In-vitro culture and rapid propagation method of lithops

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Embodiment

[0033] The present embodiment provides a method for establishing a complete tissue culture and rapid propagation system of Apricotaceae Lithops, comprising the following steps:

[0034] Step 1, Lithops explant treatment:

[0035] Select the mature leaves of the succulent plant Lithops as explants, mature female plants with a diameter of about 2-4 cm, soak the material in detergent for 15 minutes, and rinse with running water for more than 30 minutes. In the ultra-clean workbench, 75% ethanol and 2% NaClO solution were used for disinfection treatment respectively. Among them, the callus of the explants treated for 5s to 8 minutes expanded and tended to differentiate, and the sterility rate was as high as 41.2%. The rate is as high as 45.6%.

[0036] Table 1 Effects of different disinfection concentrations on callus of Lithops

[0037]

[0038] Note: Different lowercase letters indicate no significant difference at the 0.05 level.

[0039] Step 2, start cultivation:

[00...

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Abstract

The invention discloses an in-vitro culture and rapid propagation method of lithops. The method comprises the following steps: taking lithops leaves as explants, disinfecting the explants, then inoculating the explants to an initial culture medium and culturing for 3-4 weeks; inoculating the cultured and expanded explants into an induction medium and culturing for 6-7 weeks; selecting good calluses after induction culture, carrying out subculture multiplication culture, and culturing for 4 weeks; selecting strong and healthy multiple shoots after subculture multiplication culture, inoculatingthe multiple shoots into a rooting medium, culturing for 5 weeks, opening culture bottles and hardening the seedlings after the rooting rate reaches a certain number and the root length reaches the transplanting standard, then transplanting and managing tissue culture seedlings, and completing tissue culture and rapid propagation of lithops. The method is capable of rapidly and efficiently propagating lithops tissue culture seedling plants, increasing the survival rate and the rooting rate of the explants, producing a large quantity of high-quality regeneration seedlings in a short time, and improving the defects of slow growth speed and low propagation efficiency of the lithops.

Description

technical field [0001] The invention relates to the field of Lithops cultivation, in particular to a method for in vitro cultivation and rapid propagation of Lithops. Background technique [0002] Lithops sp. is a plant of the genus Lithops in the Apricot family, native to southern Africa. The metamorphic leaves of Lithops are thick, shaped like colored stones, petite and exquisite, novel and peculiar, enjoying the reputation of "living stones", and becoming one of the popular small succulents in recent years. The propagation of Lithops depends on seeds or cuttings. Cuttings can only be used for one plant to naturally form multiple heads to produce new plants, so most of them reproduce by seeds; however, the seeds are small and grow slowly, and it takes at least 3 years from germination to flowering; However, the reproduction rate of the division method is low, and it is rarely used in actual production and cultivation. [0003] In addition, people's demand for Lithops is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 田佶姚允聪陈丽张杰宋婷婷
Owner BEIJING UNIV OF AGRI
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