External pressurized capillary electrophoresis interface device and method for detecting substances
A technology of capillary electrophoresis and interface devices, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve problems affecting the detection results of substances to be detected, avoid inaccurate detection results, ensure rigidity, and improve accuracy Effect
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[0068] Using the externally pressurized capillary electrophoresis interface device of the embodiment of the present invention, substances in the liquid to be tested (separation buffer) are detected. Among them, the high-voltage power supply model used in capillary electrophoresis is HB-F303-1AC (Hengbo, Tianjin), and the detection module is a capacitively coupled non-contact conductivity detector. 1Hz~2MHz, the sine wave with the amplitude of 0-20Vp-p (peak-peak value) is the excitation signal, and the received signal is collected by the CT22 data acquisition unit (Qianpu Co., Ltd., Shanghai) via OPA627 (produced by Texas Instruments, USA). And use HW2000 chromatographic workstation (Qianpu Co., Ltd.) for processing.
[0069] Electrophoretic separation and detection conditions: The inner diameter of the separation capillary is 75 μm and the length is 70 cm. Injection time: 8s, separation voltage: 12KV. The excitation signal frequency of the non-contact conductivity detector ...
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