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External pressurized capillary electrophoresis interface device and method for detecting substances

A technology of capillary electrophoresis and interface devices, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve problems affecting the detection results of substances to be detected, avoid inaccurate detection results, ensure rigidity, and improve accuracy Effect

Inactive Publication Date: 2019-10-22
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

[0008] The embodiment of the present invention discloses an externally pressurized capillary electrophoresis interface device and a method for detecting substances, which are used to solve the technical problem that in the existing capillary electrophoresis interface device, the detection result of the substance to be detected is affected by the flow of the liquid to be tested from the interface

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  • External pressurized capillary electrophoresis interface device and method for detecting substances
  • External pressurized capillary electrophoresis interface device and method for detecting substances
  • External pressurized capillary electrophoresis interface device and method for detecting substances

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Embodiment 1

[0068] Using the externally pressurized capillary electrophoresis interface device of the embodiment of the present invention, substances in the liquid to be tested (separation buffer) are detected. Among them, the high-voltage power supply model used in capillary electrophoresis is HB-F303-1AC (Hengbo, Tianjin), and the detection module is a capacitively coupled non-contact conductivity detector. 1Hz~2MHz, the sine wave with the amplitude of 0-20Vp-p (peak-peak value) is the excitation signal, and the received signal is collected by the CT22 data acquisition unit (Qianpu Co., Ltd., Shanghai) via OPA627 (produced by Texas Instruments, USA). And use HW2000 chromatographic workstation (Qianpu Co., Ltd.) for processing.

[0069] Electrophoretic separation and detection conditions: The inner diameter of the separation capillary is 75 μm and the length is 70 cm. Injection time: 8s, separation voltage: 12KV. The excitation signal frequency of the non-contact conductivity detector ...

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Abstract

An embodiment of the invention discloses an external pressure capillary electrophoresis interface device and a method of detecting a substance, wherein the device comprises: a capillary having an interface; a sample incoming end of the capillary is connected with a liquid storage bottle, a sample outgoing end of the capillary is connected with a detection module, a pressure application spatial structure is arranged outside the interface, the interface is communicated with the pressure application spatial structure, and a liquid inlet and a negative electrode are arranged in the pressure application spatial structure; a positive electrode is arranged in the liquid storage bottle. The external pressure capillary electrophoresis interface device according to the embodiment has the advantagesthat the pressure application spatial structure is added to the outside of the interface of the capillary, a buffered liquid that is pressurized is utilized, pressure can be applied to the interface to prevent a liquid under detection in the capillary from flowing out from the interface, and detection result accuracy is improved accordingly; the external pressure capillary electrophoresis interface device according to the embodiment has no need for sealing the interface with acetic cellulose, rigidity of capillary is ensured, and the problem of detection result inaccuracy due to the fact thata substance under detection adsorbs to the acetic cellulose can be avoided.

Description

technical field [0001] The invention relates to the technical field of capillary electrophoresis, in particular to an external pressurized capillary electrophoresis interface device and a method for detecting substances. Background technique [0002] As a high-throughput separation and analysis technology, capillary electrophoresis technology is widely used in the fields of life science, bioengineering, medicine, environmental protection and food inspection. When the sample is analyzed by capillary electrophoresis technology, after the liquid to be tested is passed into the capillary, a high voltage of tens of thousands of volts is usually formed in the capillary, and the substance to be detected in the liquid to be tested moves under the action of the electric field and moves from the capillary to the capillary. The detection module is used to detect the substance to be detected. However, when detecting the substance to be detected in the capillary, the working voltage of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
Inventor 秦卫东毕俊敏
Owner BEIJING NORMAL UNIVERSITY
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