Plant root border cell silicon coating treatment method

A technology of edge cells and treatment methods, which is applied in plant cells and other directions to achieve the effect of improving the ability of anti-aluminum poisoning

Inactive Publication Date: 2018-03-09
FOSHAN UNIVERSITY
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Problems solved by technology

[0004] Many studies have shown that silicon, as a plant beneficial element, can relieve plant aluminum toxicity, and the silicon oxide deposited on the cell wall, intercellular space and in the cell duct can induce SiO due to the presence of various hydrophilic media. 2 Self-assembled to form SiO 2 Nanostructures, this deposition improves the resistance of the cell wall to external forces, however nanostructured SiO 2 How to effectively deposit in root border cells and endow plant root border cells with better aluminum toxicity resistance remains to be explored

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  • Plant root border cell silicon coating treatment method
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Embodiment

[0027] A method for treating plant root edge cells coated with silicon, comprising the following process steps:

[0028] 1) Take the root tips of 1cm plant seedlings and place them in CaCl with a concentration of 2mmol / L 2 Stir the solution with a glass rod for 1 to 2 minutes to obtain a cell collection solution;

[0029] 2) Transfer the cell collection solution to CaCl containing 0.005mg / mL polyethyleneimine 2 Solution (CaCl 2 Solution concentration is 0.5mM / L, its pH value is 7.0), incubate 10min, CaCl 2 Solution washing 2 times;

[0030] 3) Transfer the solution obtained in step 2) to a silicic acid solution (methyl orthosilicate hydrolysis solution and CaCl 2 The solution was mixed according to the volume ratio of 50:950, the pH value was 7.0), incubated for 20min, and the cell surface was siliconized with CaCl 2 solution for cleaning.

[0031] The cell collection fluid obtained from the above-mentioned embodiment was subjected to related tests of cell activity and a...

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Abstract

The invention discloses a plant root border cell silicon coating treatment method which comprises the following process steps: 1. collecting plant seedling root border cell in a CaCl2 solution to obtain a cell collection solution; 2. transferring the cell collection solution into a CaCl2 solution containing 0.005g/ml of polyethylenimine, incubating for 10min, and washing the CaCl2 solution for 1-2times; and 3. transferring the obtained solution into a silicic acid solution, incubating for 20min, silicifying the surface of the cell and then washing with the CaCl2 solution. According to the invention, a nanosilicon coating layer is formed on the surface of a plant root border cell effectively, so that technical basis is provided for improving the aluminum toxicity resistant capacity of theplant root border cell.

Description

technical field [0001] The invention relates to the field of plant cell treatment, in particular to a plant root edge cell treatment method. Background technique [0002] According to the different parts of detoxification, the physiological mechanism of plant resistance to aluminum toxicity can be divided into two categories: external rejection and internal tolerance. The former detoxification site is in the apoplast, while the latter is the symplast. The external exclusion mechanism includes the fixation of the cell wall to aluminum, the selective permeability of the plasma membrane to aluminum, the induced barrier, the secretion of chelating ligands and phosphate, and the "active transport out of the cell". The internal tolerance mechanism mainly includes The chelation of aluminum by organic acids, proteins and other organic ligands in the cytoplasm, the compartmentalization of vacuoles, the induction of anti-aluminase and the improvement of enzyme activity, etc. In the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCC12N5/04
Inventor 喻敏郭少雪李学文萧洪东刘家友冯英明
Owner FOSHAN UNIVERSITY
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