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Aspartic acid aminotransferase detection kit as well as preparation and use method thereof

An aspartate amino acid detection kit technology, applied in the field of medical testing, can solve the problems of easy reduction of enzyme activity, poor stability of the kit, inaccurate test results, etc., and achieves convenient operation, extended use and storage time. , the effect of fast detection

Inactive Publication Date: 2018-03-06
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the detection of aspartate aminotransferase is mostly carried out by using corresponding kits combined with automatic biochemical analyzers; however, the stability of the existing kits is not good, and the enzyme activity tends to decrease during long-term storage and use, resulting in the inaccurate result

Method used

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  • Aspartic acid aminotransferase detection kit as well as preparation and use method thereof
  • Aspartic acid aminotransferase detection kit as well as preparation and use method thereof
  • Aspartic acid aminotransferase detection kit as well as preparation and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] An aspartate aminotransferase detection kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0047] Reagent R1:

[0048]

[0049] Reagent R2:

[0050]

[0051] Wherein, the pH of the reagent R1 is 7.0, and the pH of the reagent R2 is 7.5.

Embodiment 2

[0053] An aspartate aminotransferase detection kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0054] Reagent R1:

[0055]

[0056]

[0057] Reagent R2:

[0058]

[0059] Wherein, the pH of the reagent R1 is 8.5, and the pH of the reagent R2 is 8.0.

Embodiment 3

[0061] An aspartate aminotransferase detection kit according to this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0062] Reagent R1:

[0063]

[0064]

[0065] Reagent R2:

[0066]

[0067] Wherein, the pH of the reagent R1 is 8.0, and the pH of the reagent R2 is 7.8.

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PUM

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Abstract

The invention discloses an aspartic acid aminotransferase detection kit which comprises a reagent R1, including trismetyl aminomethane, trismetyl aminomethane hydrochloride, disodium ethylenediamine tetraacetic acid, sodium azide, NADH (Nicotinamide-Adenine Dinucleotid), aspartic acid and G6PD, and a reagent R2, including trismetyl aminomethane, trismetyl aminomethane hydrochloride, alpha-oxoglutarate and malic dehydrogenase. The invention further discloses a preparation and use method of the aspartic acid aminotransferase detection kit. The kit has the advantages that firstly, the kit is simple to prepare, convenient to operate and low in cost, secondly, the kit is rapid in detection speed, thirdly, influence of environment factors to activity of reaction enzymes is retarded through G6PD,and thus the problems of long-term storage and degradation in the use process of the kit are solved.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an aspartate aminotransferase detection kit and a preparation method thereof. Background technique [0002] With the development of social economy and the improvement of people's living standards, human beings pay more and more attention to health. Liver disease is a common and extremely harmful disease. The timely diagnosis and treatment of liver disease play an important role in the prevention, diagnosis and treatment of the disease. In liver function tests, glutamate aminotransferase is an indicator that reflects liver cell damage, while aspartate aminotransferase is an indicator that reflects liver cell necrosis. Accurate detection of the two can accurately reflect the state of the liver . [0003] At present, the detection of aspartate aminotransferase is mostly carried out by using corresponding kits combined with automatic biochemical analyzers; however, the stab...

Claims

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Application Information

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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 吴泽东朱卫中张金东吴铮朱雨周珍珍
Owner ANHUI IPROCOM BIOTECH CO LTD
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