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High-purity konjac mannan polysaccharide as well as preparation method and application thereof

A high-purity, mannanase technology, applied in fermentation and other directions, can solve the problems of long irradiation time, increased solubility, low purity, etc., achieves important economic and production value, proliferates vaginal lactic acid bacteria, and has the effects of simple operation process

Active Publication Date: 2018-03-06
NINGBO BAIERMA BIOLOGICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] 4 Chinese patent (application number CN201310022943.X) discloses a method for the synergistic preparation of moderately polymerized konjac glucomannan. Under the premise of maintaining the original characteristics of KGM, the viscosity of KGM hydrosol is reduced, and the solubility of KGM is effectively increased. However, this method uses irradiation to degrade glucomannan, and the irradiation time is long, and each time The experimental treatment volume is small, which is not conducive to large-scale industrial production
[0005] 5 Chinese patent (application number 201110309468.5) discloses a preparation method of konjac glucomannan and glucomannan oligosaccharides with different molecular weights, and proposes to degrade glucomannan into enzymatic hydrolyzates of different molecular weights, but it is not clear The degree of polymerization of the hydrolyzate does not define the functional characteristics of the hydrolyzate of different molecular segments, and the process is simple, only after preliminary alcohol precipitation separation, the purity of the product is low

Method used

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  • High-purity konjac mannan polysaccharide as well as preparation method and application thereof
  • High-purity konjac mannan polysaccharide as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0033] 20 as figure 1 Shown a kind of preparation method of mannose, comprises the following steps:

[0034] (1) Add 200kg of production water to the enzymolysis tank, control the temperature inside the enzymolysis tank to be 30-50°C, and put neutral β-mannanase into the enzymolysis tank (the amount of enzyme input is based on the amount of enzyme and konjac extract Powder mass ratio is 1:10-30), weigh 10kg of konjac fine powder, put it evenly into the enzymolysis tank with rapid and automatic detection material viscosity device, control the pH of the material in the enzymolysis tank to 6.0-7.5, with the enzyme As the hydrolysis time advances, the viscosity of the enzymolysis liquid drops rapidly. When the viscosity real-time detection device installed on the enzymolysis tank detects that the viscosity of the enzymolysis liquid reaches 300-400mpa.s, the enzyme is immediately deactivated, and the deactivation time is 20-30min. cool down;

[0035] (2) Microfiltration to remove...

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Abstract

The invention discloses a high-purity konjac mannan polysaccharide. The high-purity konjac mannan polysaccharide is a polysaccharide system with a weight-average molecular weight of 6000-8000Da prepared by producing konjac powder by virtue of beta-mannanase controllable hydrolysis and purifying. The polysaccharide system has physiological functions of proliferating vaginal beneficial bacteria andinhibiting harmful bacteria and can be applied to antibacterial treatment of colpitis.

Description

technical field [0001] 1. The present invention belongs to the field of biochemical industry, in particular to a method for preparing high-purity konjac mannose. Background technique [0002] 2 Konjac glucomannan (KGM) is a polysaccharide formed by combining D-glucose and D-mannose through β-1, 4 glycosidic bonds, and its average degree of polymerization is between 1000 and 10000. Because KGM has defects such as high degree of molecular polymerization, high viscosity, and low solubility, the application of natural KGM is limited to a certain extent. [0003] 3 KGM can be degraded to produce glucomannan with different degrees of polymerization, among which mannan oligosaccharides are a type of sugar with a lower degree of polymerization, and the degree of polymerization of its sugar molecules is 2-10 sugars. chemical and pharmaceutical fields. [0004] 4 Chinese patent (application number CN201310022943.X) discloses a method for the synergistic preparation of moderately pol...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C08B37/02
CPCC08B37/0003C08B37/009C12P19/04C12P19/14
Inventor 钱金宏卢晓会应烨钱乾
Owner NINGBO BAIERMA BIOLOGICAL TECH CO LTD
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