Skin-whitening skin-care composition containing chrysanthemum indicum cell extract, and preparation method thereof
A technology of skin care composition and extract, which is applied in the field of whitening skin care composition containing chrysanthemum cell extract and its preparation, can solve the problems of easy inactivation and discoloration of natural tyrosinase activity inhibitors, and achieve the purpose of inhibiting the formation of melanin, Effect of controlling pigment and preventing inactivation
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Embodiment 1
[0031] An embodiment of the preparation method of the whitening skin care composition containing the chrysanthemum cell extract of the present invention, the preparation method of the whitening skin care composition containing the chrysanthemum cell extract described in this embodiment is:
[0032] (1) preparation of wild chrysanthemum cell extract
[0033] 1) Take the explants of the young shoots of the upper part of the chrysanthemum plant, wash, sterilize, inoculate on the callus induction medium, induce under dark conditions, and the temperature is 25 ° C to induce callus cells;
[0034] Wherein, induction medium is MS basal medium, adds 1-naphthaleneacetic acid (0.1mg / L), 6-benzylaminopurine (3.5mg / L), sucrose (20g / L) and agar (10g);
[0035] 2) The callus cells are continuously subcultured and proliferated in the induction medium, and the callus cell lines with uniform cell morphology and stable growth are screened;
[0036] 3) transfer the C. chrysanthemum callus cell ...
Embodiment 2
[0047] An embodiment of the preparation method of the whitening skin care composition containing the chrysanthemum cell extract of the present invention, the preparation method of the whitening skin care composition containing the chrysanthemum cell extract described in this embodiment is:
[0048] (1) preparation of wild chrysanthemum cell extract
[0049] 1) Take the explants of the young shoots of the upper part of the chrysanthemum plant, wash, sterilize, inoculate on the callus induction medium, induce under dark conditions, and the temperature is 25 ° C to induce callus cells;
[0050] Wherein, induction medium is MS basal medium, adds 1-naphthaleneacetic acid (0.1mg / L), 6-benzylaminopurine (3.5mg / L), sucrose (20g / L) and agar (10g);
[0051] 2) The callus cells are continuously subcultured and proliferated in the induction medium, and the callus cell lines with uniform cell morphology and stable growth are screened;
[0052] 3) transfer the C. chrysanthemum callus cell ...
Embodiment 3
[0063] An embodiment of the preparation method of the whitening skin care composition containing the chrysanthemum cell extract of the present invention, the preparation method of the whitening skin care composition containing the chrysanthemum cell extract described in this embodiment is:
[0064] (1) preparation of wild chrysanthemum cell extract
[0065] 1) Take the explants of the young shoots of the upper part of the chrysanthemum plant, wash, sterilize, inoculate on the callus induction medium, induce under dark conditions, and the temperature is 25 ° C to induce callus cells;
[0066] Wherein, induction medium is MS basal medium, adds 1-naphthaleneacetic acid (0.1mg / L), 6-benzylaminopurine (3.5mg / L), sucrose (20g / L) and agar (10g);
[0067] 2) The callus cells are continuously subcultured and proliferated in the induction medium, and the callus cell lines with uniform cell morphology and stable growth are screened;
[0068] 3) transfer the C. chrysanthemum callus cell ...
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