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Method for manufacturing water convolvulus root-knot nematode giant cell paraffin sections

A technology of paraffin sectioning and water spinach, which is applied in the field of making paraffin sectioning of spinach root-knot giant cells, which can solve the problems of unseen giant-cell paraffin sectioning of water spinach root knots, and achieve the effects of improving sectioning efficiency, improving dyeing effect, and ensuring section quality

Inactive Publication Date: 2018-02-16
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on paraffin section technology of giant cells in spinach root knots

Method used

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  • Method for manufacturing water convolvulus root-knot nematode giant cell paraffin sections
  • Method for manufacturing water convolvulus root-knot nematode giant cell paraffin sections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A method for making paraffin sections of spinach root knot giant cells, comprising the following steps:

[0053] (1) Take materials

[0054] Cut the collected root knots into small sections of 0.5-1cm;

[0055] (2) vacuuming

[0056] According to the size of the material, it is equipped with syringe barrels of different sizes. First, lift the pump out, hold the syringe with your left hand and hold the needle socket with your index finger, then pour in the FAA fixative and materials, insert the syringe and turn the syringe upside down so that the needle socket is facing upwards, then remove the index finger and push the pump Insert until the fixative begins to emerge from the needle hole. At this time, there are only fixative and materials in the cylinder without other gaps. Finally, press the needle hole with your index finger, and pull out the cylinder with your right hand for 2-3 times in a row. is extracted;

[0057] (3) Fixed

[0058] Put the material into a fi...

Embodiment 2

[0090] A method for making paraffin slices of spinach root knot giant cells, the method of the present embodiment is the same as that of embodiment 1, except that in step (13), two slice angles are adopted: transverse cutting and longitudinal cutting, and the slice thickness is 10 μm.

[0091] The results showed that the pear-shaped root-knot nematodes were present in the slices obtained by cross-sectioning the root-knot nematode and had a complete structure, and giant cells were distributed near the head of the root-knot nematode (see figure 2 -A); No piriform root-knot nematodes and giant cells appeared in the slice obtained by cutting the root knot longitudinally (see figure 2 -B). Therefore, the present invention finally uses a cross-section method to observe the histopathological structure of giant cells and root-knot nematodes.

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Abstract

The invention discloses a method for manufacturing water convolvulus root-knot nematode giant cell paraffin sections. According to the method, steps of staining, cross-sectioning angle, de-waxing andthe like are improved on the basis of conventional paraffin section technologies. The Ehrlich's hematoxylin staining solution is used for staining of plant tissues, and traditional dewaxing before staining is changed into mass staining before dewaxing. Section angle adopts cross-sectioning, and section thickness is 5-10 microns. According to the invention, dewaxing mode is also improved, and a TO-type biological slice-making transparent reagent is used to replace xylene for dewaxing. By the manufacturing method of water convolvulus root-knot nematode giant cell paraffin sections, staining effect of water convolvulus root-knot nematode giant cell paraffin sections is obviously enhanced, work efficiency is raised, and the effect of water convolvulus root-knot nematode giant cell paraffin sections is guaranteed.

Description

technical field [0001] The invention relates to a method for making paraffin slices, in particular to a method for making spinach root knot giant cell paraffin slices. Background technique [0002] Root-knot nematode is a serious crop nematode disease in agricultural production, which brings huge economic losses to the production of vegetables, fruit trees and food crops all over the world. Root-knot nematodes infect host plant cells, and the plant itself undergoes complex and variable pathophysiological changes, and multiple giant cells are induced in the root knot. [0003] At present, traditional methods are mainly used in the prevention and control of root-knot nematodes, which are difficult to control. With the maturity of molecular biology, the molecular interaction mechanism between root-knot nematode and its host plant giant cells should be studied in depth through molecular biology technology, and new transgenic nematode-resistant crops should be cultivated to fund...

Claims

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Application Information

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IPC IPC(8): G01N1/30
Inventor 王新荣连晓聪陈芳妮贾宁祝乐天李远孙思吴路平
Owner SOUTH CHINA AGRI UNIV
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