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Dressing for treating diabetic foot and preparation method thereof

A glycosaminoglycan and stem cell technology, applied in the field of stem cells, can solve the problems of difficult to obtain curative effect, unable to fully heal diabetic foot wound healing, etc., and achieve the effect of promoting rapid healing, reducing immune rejection, and easy to obtain.

Inactive Publication Date: 2018-02-16
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The clinical methods used to treat diabetic foot include drug therapy, vascular reconstruction, interventional surgery, ultrasonic ablation, hyperbaric oxygen therapy, negative pressure suction, wet dressing, epidermal growth factor and stem cell transplantation. The wound healing of diabetic foot cannot be completely healed, and it is difficult to achieve satisfactory curative effect, so it is urgent to find a preparation or method that can promote wound healing of diabetic foot

Method used

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preparation example Construction

[0024] The present invention has no special limitation on the source of umbilical cord mesenchymal stem cells, and those familiar to those skilled in the art can be used, such as commercially available umbilical cord mesenchymal stem cells; conventional umbilical cord mesenchymal stem cells well known to those skilled in the art can also be used The preparation method is prepared. In the present invention, the preparation method of umbilical cord mesenchymal stem cells is preferably the following specific steps:

[0025] Take the freshly collected human umbilical cord, wash the blood stains on the surface with PBS buffer, cut the umbilical cord into 1-2cm long pieces with surgical scissors, remove the arteriovenous and adventitia respectively, and cut to (20-50)mm with ophthalmic scissors 3 size organization block.

[0026] Flatten an appropriate amount of tissue pieces into a Petri dish with tweezers, add to DMEM / F12 culture medium containing 10% FBS, and transfer to 5% CO ...

Embodiment 1

[0043] Step 1 Isolation of human umbilical cord mesenchymal stem cells

[0044] Take the freshly collected human umbilical cord, wash the blood stains on the surface with PBS buffer, cut the umbilical cord into 1-2cm long pieces with surgical scissors, remove the arteriovenous and adventitia respectively, and cut to (20-50)mm with ophthalmic scissors 3 size organization block.

[0045] Flatten an appropriate amount of tissue pieces into a Petri dish with tweezers, add to DMEM / F12 culture medium containing 10% FBS, and transfer to 5% CO 2 , 37°C, and a saturated humidity of 95% in an incubator, the first 5-7 days are static culture, and the first rehydration is performed when the color of the culture solution changes to light yellow; when at least 5 places crawl out of each dish are observed After forming a sheet of cells, use a pipette to suck off the culture medium and tissue pieces in the dish, and then perform liquid replacement treatment to continue culturing, and then ob...

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Abstract

The application belongs to the technical field of stem cell and specifically relates to dressing for treating diabetic foot and a preparation method thereof. The dressing for treating diabetic foot isan effective combination of multiple ingredients such as stem cell growth factors, umbilical cord mesenchymal stem cells, fibroblast and the like. Through compatibility and synergistic interaction ofthe ingredients, wound healing of diabetic foot is promoted. The invention also provides a preparation method of the dressing. Steps are simple to operate, the process is optimized, and large-scale production can be achieved.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to a dressing for treating diabetic foot and a preparation method thereof. Background technique [0002] With social progress and changes in people's lifestyles and diets, the incidence of diabetes is increasing year by year. According to a recent epidemiological study in China, the prevalence of diabetes in China has reached 9.7%, which is higher than the world average of 6.4%. Among them, diabetic foot is the most serious complication of diabetes and often leads to non-traumatic amputation. With the development of population aging in our country, the incidence of diabetic foot is increasing year by year. Improper treatment may lead to amputation or death, and 85% of amputations are caused by ulcers. The occurrence of diabetic foot ulcers reduces the quality of life of diabetic patients, prolongs the hospitalization time, and brings a huge economic burden to societ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0033A61L26/0066A61L26/008A61L2300/252A61L2300/30A61L2300/412A61L2300/414C08L89/00C08L5/08
Inventor 葛啸虎陈海佳王一飞梁美乐王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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