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Preservation solution for erythrocyte

A preservation solution and red blood cell technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of interfering with the interpretation of experimenters, easily destroying red blood cells, and limited application, so as to reduce false positives, column marks, and so on. Effect of reducing low temperature damage and solving aggregation phenomenon

Active Publication Date: 2018-02-16
SICHUAN MACCURA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complicated operation of cryopreservation and the fact that red blood cells are easily damaged during the cryopreservation process and rethawing process, the application of cryopreservation is currently limited.
[0004] At present, the main manufacturers of commercial red blood cell kits on the market include Shanghai Blood Biomedicine Co., Ltd., Jiangyin Libo Pharmaceutical Biotechnology Co., Ltd., and Changchun Boxun Biotechnology Co., Ltd.; foreign manufactu

Method used

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  • Preservation solution for erythrocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Screening and Determination of Carbohydrates in Erythrocyte Preservation Solution

[0045] (1) Selection of carbohydrates Carbohydrates are an essential component in the preservation of red blood cells. They provide energy for the metabolism of red blood cells in vitro, and play a significant role in maintaining the shape and physiological functions of red blood cells. The screening scheme of this experiment is shown in Table 3. Based on the basic formula in Table 1, sugars were added, and no anticoagulant and other components were added.

[0046] table 3

[0047] Program

components

concentration

plan 1

glucose

20g / L

Scenario 2

Trehalose

20g / L

Option 3

sucrose

20g / L

Option 4

Glucose+Trehalose

10g / L+10g / L

Option 5

Trehalose + sucrose

10g / L+10g / L

Option 6

Sucrose + Glucose

10g / L+10g / L

[0048] The test results are shown in Table 4. It can be found th...

Embodiment 2

[0064] Screening and determination of embodiment 2 anticoagulants

[0065] (1) Screening of anticoagulants The effect of adding anticoagulants is mainly to prevent the non-specific aggregation of red blood cells. In the test, the applicant found that the amount of anticoagulants used in the red blood cell preservation solution was appropriately increased for use in microcolumns. When the gel method is used for pre-transfusion inspection items (blood type detection, irregular antibody screening, cross-matching), it can effectively reduce the phenomenon of column imprint, scattered red blood cell agglutination, tailing, etc. that interfere with the interpretation of experimental results. The screening scheme of this experiment is shown in Table 7 below.

[0066] Table 7

[0067] Program

components

concentration

plan 1

heparin

5g / L

Scenario 2

sodium citrate

5g / L

Option 3

EDTA-Na 2

5g / L

[0068] The experimenta...

Embodiment 3

[0078] Example 3 Screening and Determination of pH Value of Erythrocyte Preservation Solution

[0079] The pH value has a great influence on the preservation of red blood cells in vitro. It not only affects the metabolism of red blood cells, but also directly affects the oxygen dissociation curve. The inventors found that the storage of red blood cells in an acidic preservation liquid will accelerate the hemolysis of red blood cells, and with the extension of storage time, red blood cells The color of the erythrocyte gradually turns dark purple, which may be due to the low pH, which makes the oxyhemoglobin in the red blood cells easy to dissociate and release oxygen. Variety. The screening scheme and experimental results of this embodiment are shown in Table 10.

[0080] Table 10

[0081]

[0082]

[0083]

[0084]

[0085] Through experiments, it can be found that when the pH value of the red blood cell preservation solution is acidic, as the storage time prolon...

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Abstract

The invention provides a preservation solution for an erythrocyte. The preservation solution is prepared from the following raw materials with concentrations: 10.0g/L to 40.0g/L disaccharide, 1.0g/L to 3.0g/L sodium chloride, 3.0g/L to 10.Og/L EDTA-Na2 (Disodium Ethylenediaminetetraacetate), 5.0g/L to 10.0g/L glycine, 0.1g/L to 0.3g/L of KH2PO4, 5g/L to 10g/L Na2HPO4.12H2O, 30mg/L to 90mg/L of sulfanilamide, 10mg/L to 30mg/L trimethoprim and 0.15g/L to 1.3g/L antioxidant, wherein the pH (potential of Hydrogen) is 7.5 to 8.5; the osmotic pressure is 240mosm to 380mosm. The preservation solutionfor the erythrocyte, which is provided by the invention, can be used for obviously decreasing the hemolysis ratio of the erythrocyte during preservation, is used for prolonging the preservation timeof the erythrocyte and can be used for avoiding the aggregation phenomenon of the erythrocyte during in-vitro preservation and the preservation can reach 120 days at 2 to 8 DEG C.

Description

technical field [0001] The invention relates to the technical field of preparation of biochemical reagents, in particular to a preservation solution for preserving red blood cells in vitro and its application. Background technique [0002] The blood type serological test before blood transfusion is the most basic and important experimental item to ensure the safety of blood transfusion. Antigen-antibody reactions are inseparable from blood group serology experiments. At present, the antibody reagents used in blood group serology have already been commercialized and standardized in large quantities due to their convenient production and purification. As another essential component in the reaction system is red blood cells, the problems it faces as a reagent are much more complicated. First of all, the raw material of red blood cells must come from the human body and cannot be replaced by substances from other sources. Secondly, the life span of red blood cells is short, wit...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 毛亚琳王小波
Owner SICHUAN MACCURA BIOTECH CO LTD
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