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Chicken specialized intestinal occupation bacteria and production method and application thereof

A technology of space-occupying bacteria and space-occupying bacteria, applied in the field of microecology, to achieve the effect of inhibiting pathogenic bacteria invasion, strong colonization ability, and broad application prospects

Inactive Publication Date: 2018-02-02
JINAN SANFENG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no report of chick-specific intestinal space-occupying bacteria consisting of maternal cecum-adhesive beneficial bacteria flora that competes for intestinal epithelial cell adsorption sites through steric hindrance

Method used

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  • Chicken specialized intestinal occupation bacteria and production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Preparation of seed solution of intestinal space-occupying bacteria agent

[0022] Select 22±2 day-old healthy hens fed without antibiotics, after slaughter, take the cecum part and cut it open, rinse the contents with sterile saline, wash it twice, and place it in a lactic acid bacteria medium (MRS ), after anaerobic enrichment culture at 37°C for 30±6 hours, the culture solution obtained is the seed solution of the intestinal space-occupying bacteria agent.

[0023] Among them, the composition of MRS medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 0.5g sodium acetate, 2.0g diamine citrate, 1ml Tween 80, 2.0g dipotassium hydrogen phosphate, 0.2g magnesium sulfate, 0.05g manganese sulfate, 1000ml distilled water, pH6.8.

Embodiment 2

[0024] Example 2: Culture of Beneficial Bacteria Cells in Intestinal Occupying Bacteria Agent

[0025] Inoculate the seed liquid with 5% inoculation amount into a 1 ton reactor pre-loaded with 2 / 3 volume of GS medium, and after anaerobic cultivation at 37°C±1°C for 22±2 hours, test the obtained fermentation liquid, when it When the pH value is lowered to 4.0, it can be used to prepare beneficial bacteria cells.

[0026] Wherein, the GS medium composition is: 15g glucose, 5g starch, 8g soybean protein powder, 5g yeast extract powder, 0.6g L-cysteine, 5 sodium acetate, 0.4g magnesium sulfate, 0.05g calcium chloride, Add water to 1000 mL, pH 6.8.

Embodiment 3

[0027] Example 3: Preparation of intestinal space-occupying bacteria powder

[0028] (1) The fermented liquid obtained in Example 2 is centrifuged at 8000 ± 500 rpm / min for 5 ± 1 min, and the precipitation is the beneficial bacterial group cells;

[0029] (2) Using the conventional serial dilution coating plate method, the obtained intestinal space-occupying bacteria powder is counted on the plate, and when the total effective number of beneficial bacteria cells is detected, it is not less than 5×10 9 For CFU / g, mix the cells according to the ratio of 5 g of beneficial bacteria cells per liter of the protective agent, and routinely freeze-dry to prepare the special intestinal space-occupying bacteria powder for chicks.

[0030] Wherein, the above protective agent solution contains: 15g glycerin, 100g skim milk, 80g starch, 1000mL water.

[0031] (3) Metagenome sequencing and analysis found that the beneficial bacteria contained in the obtained intestinal space-occupying agent...

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PUM

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Abstract

The invention discloses chicken specialized intestinal occupation bacteria and a production method and application thereof. The intestinal occupation bacteria is prepared by evenly mixing of beneficial bacterial cells attached to the surface of cecum mucosa of a healthy hen and a protective agent prior to freeze drying, the proportion of the beneficial bacterial cells and the protective agent is 5-10g of the beneficial bacteria cells in per liter of the protective agent, and the total effective number of the bacteria is not less than 5X10<9>CFU / g. The invention further discloses the application of the intestinal occupation bacteria in improving immunity and disease resistance of chicken. Experimental results show that intestinal occupation bacterial is diversified and easy to colonize andsurvive in intestines of the chicken, shortcomings that conventional microecological preparations are poor in host specificity, singular in variety and the like are overcome, and broad application prospect in large-scale production of livestock and poultry farming is achieved.

Description

technical field [0001] The invention relates to a micro-ecological agent for livestock and poultry breeding and its preparation method and application, in particular to a special intestinal space-occupying bacterial agent for chicks and its preparation method and application, belonging to the technical field of micro-ecology. Background technique [0002] The intestinal tract is an important immune organ of the body, and the distribution of intestinal microbial flora is related to the health level of animals. A perfect composition of intestinal flora can give the body a healthy level; an imbalance of intestinal flora can easily cause animal diseases. The composition of the intestinal flora is very complex, and the composition of the flora varies among different animals. The intestinal tract of newborn young animals is sterile, and the intestinal flora is gradually established and perfected during the process of breathing and eating. The distribution of chicken intestinal f...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61K35/744A61P37/04A61P31/04C12N1/20C12R1/225C12R1/01
Inventor 杨鹏张荣岭李世旭孔健
Owner JINAN SANFENG BIOLOGICAL ENG CO LTD
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