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Cardiovascular disease personalized drug related gene polymorphism detection system and kit

A genetic detection and cardiovascular technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve problems such as poor repeatability, few detection sites, and high detection costs

Active Publication Date: 2021-02-19
BEIJING MICROREAD GENE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These detection methods have defects of various degrees, such as cumbersome operation process, poor repeatability, difficult interpretation of results, few detection sites, low detection throughput, long detection cycle, high detection cost, etc.

Method used

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  • Cardiovascular disease personalized drug related gene polymorphism detection system and kit
  • Cardiovascular disease personalized drug related gene polymorphism detection system and kit
  • Cardiovascular disease personalized drug related gene polymorphism detection system and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0174] Example: Detection of blood and DNA samples with a detection kit for genetic polymorphisms related to personalized medicine for cardiovascular diseases 1. Detection system

[0175] The kit includes PCR Master Mix, positive control, negative control, and internal standard. The main components of PCR Master Mix include hot start DNA Taq enzyme, UDG enzyme, amplification buffer and primers for each site, etc.

[0176] According to the principle of allele characteristic PCR, each specific primer can only be combined with the DNA template of the corresponding genotype and amplified. For this purpose, a series of specific changes or modifications were made to each primer. In order to coordinate amplification efficiency, improve product peak shape, and facilitate capillary electrophoresis detection, a series of specific changes or modifications were also made to the primers. The improved and optimized primer sequences used in this example are as follows:

[0177] ACE gene i...

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Abstract

The invention discloses a detection system and a kit for detecting the polymorphism of genes related to personalized medicine for cardiovascular diseases. This system involves quantitative fluorescent PCR amplification (Quantitive Fluorescent PCR, QF‑PCR) technology and capillary electrophoresis detection technology. Features of the system: 1. Through multiplex PCR, 27 polymorphic sites on 17 genes can be amplified in one tube. 2. The test results can provide references for the use of commonly used drugs such as antihypertensive drugs, antiplatelet drugs, oral anticoagulants, and lipid-lowering drugs. 3. This system can realize direct amplification of blood and blood card samples, eliminating the step of DNA extraction; 4. This system can integrate UDG‑dUTP anti-pollution measures, which can effectively prevent product contamination. The invention has the advantages of comprehensive coverage of detection sites, high specificity, high sensitivity, strong reliability, simple operation, strong practicability, low cost, and the ability to detect in large quantities.

Description

technical field [0001] The invention relates to a multiple fluorescent PCR technology and capillary electrophoresis technology. At the same time, the polymorphisms of 27 gene loci related to personalized medicine for cardiovascular diseases were detected, which provided a reference for personalized medicine in the treatment of cardiovascular-related diseases in clinical practice. It belongs to the clinical molecular diagnosis detection technology in the field of biomedicine. Background technique [0002] The incidence of cardiovascular disease is increasing year by year all over the world, and has become the number one killer affecting human health. However, due to individual differences, the therapeutic effects of the drugs commonly used in clinical practice for cardiovascular diseases are obviously different for different patients taking the same dose of drugs, which makes clinicians have certain troubles when using the drugs for treatment. More and more studies have fou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6883C12N15/11
Inventor 张奇刘朝晖张晔周洲陈初光
Owner BEIJING MICROREAD GENE TECH
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