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Production technology for producing pulullan by fermenting aureobasidium pullulans

A technology of Aureobasidium pullulans and pullulan, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of high energy consumption, long fermentation cycle, etc., achieve reduced production costs, convenient sources, and save raw material costs Effect

Inactive Publication Date: 2018-01-30
北京艾普希隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The highest yield is 14.37g / L; the conversion rate is only 47.9%; the fermentation period is long, 144-168h; the fermentation medium contains 5.0g / L peptone, 5.0g / L rice bran, and 1.0g / L oxalic acid; 121 ℃ High temperature and high pressure steam sterilization for 20 minutes, high energy consumption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Aureobasidium pullulans strain slanted single bacterium colony is connected in the first-level seed shake flask and carries out shake flask activation, and the first-grade seed shake flask is made in the Erlenmeyer flask of packing into 250ml by 30ml first-grade seed culture medium, and rotating speed is 250rpm, and temperature is Cultivate at 28°C until OD600=4.0. The seed culture solution after activation is connected in the amount of 5% (v / v) in new secondary seed shaking flask (the secondary seed culture medium of 80ml of 500ml Erlenmeyer bottle) and carries out expansion cultivation, and rotating speed is 250rpm, and temperature is 28 Cultivate until OD600=6.5. Gained seed liquid after expanded cultivation is connected in the amount of 5.0% (v / v) in three-stage seed shaking flask (the three-stage seed culture medium of 800ml of the triangular flask of 3000ml) and cultivates, stirring speed control 200rpm, temperature is 28 ℃ , cultivated to OD600=25 to obtain the ...

Embodiment 2

[0047] Aureobasidium pullulans strain slanted single bacterium colony is connected in the first-level seed shake flask and carries out shake flask activation, and the first-grade seed shake flask is made in the Erlenmeyer flask of packing into 250ml by 30ml first-grade seed culture medium, and rotating speed is 250rpm, and temperature is Culture at 28°C until OD600=3.5. The seed culture solution after activation is connected in the amount of 5.0% (v / v) in new secondary seed shaking flask (the secondary seed culture medium of 80ml of the Erlenmeyer bottle of 500ml) and carries out expansion cultivation, and rotating speed is 250rpm, and temperature is 28 °C, culture OD600=8.0. Gained seed liquid after expanded cultivation is connected in the amount of 5.0% (v / v) in three-stage seed shaking flask (the three-stage seed culture medium of 800ml of the triangular flask of 3000ml) and cultivates, stirring speed control 200rpm, temperature is 28 ℃ , cultivated to OD600=32 to obtain t...

Embodiment 3

[0050] Aureobasidium pullulans strain inclined-plane single colony is connected in the first-level seed shake flask and shakes flask activation, and the first-grade seed shake flask is made in the Erlenmeyer flask that packs into 250ml by 30ml first-grade seed culture medium, and rotating speed is 150rpm, and temperature is Cultivate at 30°C until OD600=2.5. The seed culture solution after activation is connected in the amount of 15.0% (v / v) in new secondary seed shaking flask (the secondary seed culture medium of 80ml of the Erlenmeyer bottle of 500ml) and carry out expansion cultivation, and rotating speed is 150rpm, and temperature is 30 °C, culture OD600=9.0. Gained seed liquid after expanded cultivation is connected in the amount of 15.0% (v / v) in the three-stage seed shaking flask (the three-stage seed culture medium of 800ml of the Erlenmeyer bottle of 3000ml) and cultivates, stirring speed control 150rpm, temperature is 30 ℃ , cultivated to OD600=20 to obtain the cult...

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Abstract

The invention discloses a production technology for producing pulullan by fermenting aureobasidium pullulans. The production technology comprises the following steps: culturing seeds; preparing a seedculture medium; preparing a potato dextrose agar culture medium, a first-grade seed culture medium, a second-grade seed culture medium and a third-grade seed culture medium respectively; carrying outactivated culture on the seeds; inoculating an aureobasidium pullulans strain slant single bacterial colony to a first-grade seed shake flask and carrying out shake flask activation; inoculating an activated seed culture solution into a new second-grade seed culture shake flask according to the volume percent concentration of 1 percent to 20 percent and carrying out amplified culture; then inoculating the seed culture solution subjected to the amplified culture into a third-grade seed shake flask according to the volume percent concentration of 1 percent to 20 percent and culturing to obtaina seed culture solution to be fermented; inoculating the seed culture solution to be fermented into a fermentation tank filled with a fermentation culture medium and carrying out fermentation cultureto obtain the pulullan. The production technology disclosed by the invention has the advantages that the source of the raw materials is wide and the production cost is reduced, and has the effects ofeconomical efficiency, high efficiency and energy saving.

Description

technical field [0001] The invention relates to the technical field of pullulan production, in particular to a production process for producing pullulan by fermentation of Aureobasidium pullulans. Background technique [0002] The production of pullulan by microbial fermentation was first discovered in 1959 by German scientist Bender. In 1992, Nagase Company of Japan (formerly Hayashibara Company of Japan) industrialized the production of pullulan by fermenting Aureobasidium pullulans. Pullulan is a viscous exopolysaccharide produced by Aurebasidium pullulans by aerobic fermentation of glycosylated compounds. Linear polymer polysaccharides formed by repeated glycosidic linkages. The molecular weight is generally between 4.8×104-2.2×106Daltons, the average molecular weight is 2×105Daltons), the degree of polymerization is 100-5000, and its optical rotation is 192° at a concentration of 10g / L. Pullulan is a non-ionic, non-reducing stable polysaccharide, which is infinitely ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/10C12N1/14C12R1/645
Inventor 晋玉宽王亚飞
Owner 北京艾普希隆生物科技有限公司
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