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Reagent or kit for evaluating intestinal lipid accumulation and its intervention, and application and detection method thereof

A lipid accumulation and kit technology, applied in the fields of biochemistry, medicinal chemistry, and analytical chemistry, can solve problems such as poor resolution, achieve high sensitivity, simple operation, and improve quantitative accuracy.

Active Publication Date: 2018-01-26
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Claims
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Problems solved by technology

In addition, liquid chromatography-mass spectrometry is less capable of resolving the positions of unsaturated bonds in liquid-isolated fatty acids and monoglyceride isomers than gas chromatography-mass spectrometry
There is no report on the use of oleic acid and 1-monoolein combined marker kit to evaluate intestinal lipid accumulation and its intervention effect

Method used

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  • Reagent or kit for evaluating intestinal lipid accumulation and its intervention, and application and detection method thereof
  • Reagent or kit for evaluating intestinal lipid accumulation and its intervention, and application and detection method thereof
  • Reagent or kit for evaluating intestinal lipid accumulation and its intervention, and application and detection method thereof

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Embodiment

[0056] The present invention will be further described below in conjunction with examples, but the examples are only used to illustrate the present invention, rather than limit the present invention. Those skilled in the art can also make various similar modifications or substitutions without departing from the method and idea of ​​the present invention, and such similar modifications or substitutions are all included within the scope defined by the claims of the present application.

[0057] 1. Establishment of mouse model

[0058] ①Normal group: 5-week-old common C57BL / 6J mice (6 mice) were fed with normal mouse diet (Nanjing Junke Bioengineering Co., Ltd.) every day for 16 weeks after adaptive feeding for 1 week. ②Lipid accumulation group: 5-week-old APOE - / - Mice (C57BL / 6J, 7 mice) were fed with high-fat diet (10.0% lard + 4.0% milk powder + 2.0% cholesterol + 0.5% sodium cholate + common mouse diet) every day after adaptive feeding for 1 week, Nanjing Junke Bioengineeri...

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Abstract

The invention relates to a new application of oleic acid and glycerol 1-monooleate in the preparation of a kit for evaluating the intestinal lipid accumulation in a tested organism and its intervention effect, and a detection method of the oleic acid and the glycerol 1-monooleate. A reagent and the method are used to detect the content of the oleic acid and the glycerol 1-monooleate in the intestinal tissues of the tested organism. The invention further provides the method for detecting the content of the oleic acid and the glycerol 1-monooleate in the intestinal tissues of the tested organismthrough gas chromatography-mass spectrometry. The content can be used for evaluating the intestinal lipid accumulation and its intervention effect. The kit and the detection method have the characteristics of simplicity in operation, good reliability, fastness, high sensitivity, high specificity, and easiness in promotion.

Description

technical field [0001] The invention relates to the new application of oleic acid and 1-monoolein in the preparation of reagents or kits and detection methods for evaluating intestinal lipid accumulation and its intervention effect, and belongs to the fields of analytical chemistry, biochemistry and medicinal chemistry. technical background [0002] With the development of the economy and the change of lifestyle, the prevalence of risk factors for cardiovascular disease in my country is becoming more and more obvious, leading to a continuous increase in the morbidity and mortality of cardiovascular disease. At present, cardiovascular disease accounts for more than 40% of residents' death from diseases, and is the first cause of death among residents. It has become a major public health problem, and its prevention and treatment is urgent. Cardiovascular disease is closely related to lifestyle, especially diet, smoking, exercise and psychological stress. According to the Worl...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 叶国注董四君林怡朱慧敏
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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