Gynura formosana kitam. total flavone extract, preparation method and anti-inflammatory and analgesic use thereof

A technology of total flavonoids and extracts, applied in the directions of anti-inflammatory agents, food science, drug combination, etc., can solve the problems of no anti-inflammatory and analgesic effects of cabbage, no anti-inflammatory and analgesic effects of cabbage extracts, etc.

Inactive Publication Date: 2018-01-26
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, there is no relevant report on the anti-inflammatory and analgesic effect of Baifengcai in the prior art, and there is no relevant report on the anti-inflammatory and analgesic effect of Baifengcai extract.

Method used

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  • Gynura formosana kitam. total flavone extract, preparation method and anti-inflammatory and analgesic use thereof
  • Gynura formosana kitam. total flavone extract, preparation method and anti-inflammatory and analgesic use thereof
  • Gynura formosana kitam. total flavone extract, preparation method and anti-inflammatory and analgesic use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this example, the total flavonoid extract of Baifengcai was prepared according to the following method:

[0043] (1) Extraction: Take 100 g of Baifengcai, add 30 times the weight of water for extraction, adjust the pH of the extract to 5 with dilute hydrochloric acid to obtain a reaction liquid;

[0044] (2) Enzymatic hydrolysis: Then add 25g of complex enzyme (the complex enzyme is composed of papain, cellulase and pectinase in a weight ratio of 1:3:2) to the reaction solution for enzymatic hydrolysis, and forced circulation reaction at 40°C After 3 hours, filter with suction and collect the filtrate;

[0045] (3) Extraction and concentration: add the filtrate to an extraction tank containing AB-8 macroporous resin, stir for 12 hours at 30°C and 100 rpm, filter, and take the weight of AB-8 macroporous resin after adsorption Add 20 weight times of the AB-8 macroporous resin to 75% ethanol solution, then stir for 12 hours at 30°C and 120 rpm and filter to obtain the extract...

Embodiment 2

[0063] In this example, the total flavonoid extract of Baifengcai was prepared according to the following method:

[0064] (1) Extraction: Take 100g of Baifengcai, add 20 times the weight of water for extraction, adjust the pH of the extract to 8 with dilute sodium hydroxide solution to obtain a reaction liquid;

[0065] (2) Enzymatic hydrolysis: Then add 20g of complex enzyme (the complex enzyme is composed of papain, cellulase and pectinase in a weight ratio of 0.5:5:1) to the reaction solution for enzymatic hydrolysis, and forced circulation reaction at 30°C After 4 hours, filter with suction and collect the filtrate;

[0066] (3) Extraction and concentration: add the filtrate to an extraction tank containing DM-130 macroporous resin, stir at 30°C and 80 rpm for 24 hours, filter, and take the weight of DM-130 macroporous resin after adsorption. DM-130 macroporous resin was added 10 weight times of ethanol solution with a volume concentration of 95%, then stirred at 30°C and 80 rp...

Embodiment 3

[0071] In this example, the total flavonoid extract of Baifengcai was prepared according to the following method:

[0072] (1) Extraction: Take 100g of Baifengcai, add 60 weight times of water for extraction, adjust the pH of the extract to 4 with dilute hydrochloric acid to obtain a reaction liquid;

[0073] (2) Enzymatic hydrolysis: Then add 32g of complex enzyme (the complex enzyme is composed of papain, cellulase and pectinase in a weight ratio of 1.5:2:3) to the reaction solution for enzymatic hydrolysis, and forced circulation reaction at 50°C For 1 hour, filter with suction and collect the filtrate;

[0074] (3) Extraction and concentration: add the filtrate to the extraction tank containing ZH-01 macroporous resin, stir at 30°C and 150rpm for 6 hours, filter, and take the weight of ZH-01 macroporous resin after adsorption Add 30 weight times of the ZH-01 macroporous resin to a 70% ethanol solution with a volume concentration of 70%, then stir at 30°C and 150rpm for 6 hours, ...

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Abstract

Belonging to the field of drugs or health care products, the invention in particular relates to a gynura formosana kitam. total flavone extract, a preparation method and anti-inflammatory and analgesic use thereof. The extract contains, by weight percentage, 80-85% of rutin. The experimental result of mouse ear swelling experiments and carrageenan induced rat foot swelling experiments shows that the extract has certain anti-inflammatory effect; the experimental result of mouse hot plate experiments and acetic acid writhing experiments show that a high and middle dose of the extract can reach good analgesic effect, therefore, the gynura formosana kitam. total flavone extract provided by the invention can be used as a potential anti-inflammatory and analgesic drug. According to the preparation method of the gynura formosana kitam. total flavone extract, after an extraction step, a compound enzyme composed of specific enzymes in specific proportion is selected for enzymolysis, and furtherthrough macroporous resin extraction concentration and macroporous resin separation and purification steps, the HPLC purity of rutin in the prepared gynura formosana kitam. total flavone extract canreach 80-85%.

Description

Technical field [0001] The invention belongs to the field of medicines or health products, and specifically relates to an extract of total flavonoids of Baifengcai, a preparation method thereof, and an anti-inflammatory and analgesic application. Background technique [0002] Inflammation is a defensive response of the body to adverse external stimuli. In the process of inflammation, various inflammatory factors such as prostaglandin, serotonin, bradykinin, etc., can directly or indirectly cause tissue and cell destruction, causing pain, and local swelling of inflammatory tissue due to hyperemia and increased exudate , Compressing nerve endings also causes pain. Usually inflammation caused by infection is the body's defensive response and is beneficial to a certain extent. However, certain allergic reactions such as allergic rhinitis, urticaria, anti-basement membrane glomerulonephritis and autoimmune diseases such as ulcerative colitis, rheumatoid arthritis, systemic lupus ery...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P29/00A23L33/105A61K31/7048
Inventor 曾金香庄毅超于娟罗志毅杨小平林锦霞
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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