Single copy library construction kit

Abstract

The invention provides a single copy library construction kit, and in particular relates to the technical field of gene library construction. The single copy library construction kit comprises a reagent strip and reagents needed by library construction, wherein a plurality of storage holes are formed in the reagent strip; each storage hole sequentially comprises a bottom, a lower part and an upperpart, wherein the bottom is closed; the lower parts of the plurality of storage holes are independent of one another, and the upper parts of the every two adjacent storage holes are connected with each other; the reagents needed by the library construction are respectively placed in the storage holes. The single copy library construction kit only treats one sample, thus reducing the enzymatic activity loss, caused by repeated freezing and thawing, of tool enzyme; the single copy library construction kit eliminates the risk of inconsistency, caused by wall adhesion of liquid reagents in a storage and transportation process, between the actual consumption and the theoretical consumption of the liquid reagents; the single copy library construction kit minimizes the manual operation steps ofan operator, reduces the probability of misoperation, reduces the training demand on the operator, and guarantees sample preparation quality and reliability.

Description

technical field [0001] The invention belongs to the technical field of gene library construction, and in particular relates to a single-person library construction kit. Background technique [0002] Regardless of the genetic testing technique, DNA sample preparation is required, and sequencing also requires library preparation. High-quality sequencing library preparation is a key factor in the integrated sequencing process, and the quality of the library determines the quality of the sequencing. It is predicted that with the development of the sequencing market, library preparation will account for 15% of the molecular diagnostics market, and the market size will reach 4 billion yuan by 2025. [0003] Gene libraries include genome libraries and partial gene libraries. Many DNA fragments containing different genes of a certain organism are introduced into a population of recipient bacteria for storage, and each recipient bacteria contains different genes of this organism, w...

AI Technical Summary

Problems solved by technology

[0004] Existing library construction kits are generally stored in reagent bottles and can be used for library construction of multiple samples. When used, due to different operations by different people, there may be large errors. The tool enzymes used are repeatedly frozen and thawed. The activity of the gene library decreases, which affects the experimental effect, and finally makes the difference of the obtained gene library relatively large.

In addition, the actual amount of liquid reagents attached to the wall during storage and transportation is inconsistent with the theoretical amount. Therefore, more liquid reagents must be added during production to ensure the actual amount of reagents used by customers, which virtually increases the production cost of the enterprise.

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