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Daphnis nerii viral pesticide and preparation method thereof, and artificial feed and application thereof

A technology of oleander hawkmoth and virus insecticide, which is applied in the directions of pesticides, botanical equipment and methods, biocides, etc., and can solve the problem of no oleander hawkmoth virus oleander hawkmoth virus insecticide. To avoid the problems of pesticides, sustainable control of unfavorable pests, damage to ecological balance, etc., to achieve the effect of not easy to drug resistance, conducive to sustainable control and maintenance of ecological balance

Pending Publication Date: 2017-12-26
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, malathion, deltamethrin and organophosphorus chemical pesticides are still the main methods of controlling hawkmoth pests in my country. At the same time, it also harms the natural enemies of pests and destroys the ecological balance. At the same time, it is easy to cause resistance of pests, which is not conducive to the sustainable control of pests.
[0006] However, there is no relevant research on oleander hawkmoth virus and no effective insecticide products for the control of oleander hawkmoth virus

Method used

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  • Daphnis nerii viral pesticide and preparation method thereof, and artificial feed and application thereof
  • Daphnis nerii viral pesticide and preparation method thereof, and artificial feed and application thereof
  • Daphnis nerii viral pesticide and preparation method thereof, and artificial feed and application thereof

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preparation example Construction

[0036] The oleander hawkmoth virus insecticide provided in this embodiment can be prepared by any production method, and this application provides a simple preparation method, which mainly includes the following steps:

[0037] Step 1: Larva rearing, inserting the larvae into a feeding board equipped with oleander hornworm artificial feed, and putting them into a feeding room for feeding; this feeding can be carried out in any environment, and the feeding environment here is as follows:

[0038]Breeding room (temperature control, humidity control, light control) should reach the cleanliness level of 1000, preferably ≧0.5μm dust ≦35 grains / L; ventilation effect, light intensity should reach 3000LX above, and install ultraviolet lamp.

[0039] Step 2: virus infection, re-prepare a feeding plate, raise the oleander hawkmoth larvae to the 4th instar under the environment of step 1, throw the above-mentioned oleander hawkmoth feed into a new feeding plate, and Connect the oleander ...

Embodiment 1

[0054] The insecticide preparation method in the present embodiment is:

[0055] 1. Place 100 oleander hornworm pupae in a container (incubator) at 24°C for eclosion, put the eclosioned adults into the container to mate and lay eggs, and put 2 pairs of adults in each container ; Finally, the larvae are hatched after the above-mentioned eggs are routinely sterilized;

[0056] 2. Put the uniformly heated oleander hornmoth artificial feed into the feeding plate in advance, and insert the 1st instar larvae, and put it into the breeding room for feeding. The feeding temperature is 24 °C; the formula of the artificial feed used is shown in Table 1 Recipe 1 in ;

[0057] 3, the larvae are raised to 4 instars, the oleander hornworm is thrown feed and packed in another feeding plate, and the suspension dimension 2,000,000 PIB / ml virus suspension is inserted on its surface (this virus suspension is the , preferred nucleopolyhedrosis virus, adding an appropriate amount of distilled wat...

Embodiment 2

[0061] The steps of this embodiment are the same as those of Embodiment 1, and the difference is that:

[0062] 1. The number of oleander hawkmoth pupae is 500;

[0063] 2. The formula of the artificial feed used for raising oleander hornworm larvae is formula 2 in Table 1;

[0064] 3. The virus suspension inserted in step 3 is 1 million PIB / ml;

[0065] 4. The weight ratio of the components added in step 5 is different. The weight ratio used in this embodiment is: glycerin 1.5%, nano-scale silicon dioxide 0.2%, refined fluorescent whitening agent 0.8%, aromatic amino acid 0.14%, sodium lignosulfonate 0.3%, polyvinyl alcohol 1%, chlorfluazuron 0.06%, and the balance in sterile water.

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Abstract

The invention discloses daphnis nerii viral pesticide and a preparation method thereof, and artificial feed and application thereof. By adopting the pesticide provided by the invention, influences on afforestation are reduced and the problem of killing natural enemies of daphnis nerii when the daphnis nerii are killed is solved; the artificial feed provided by the invention is equivalent to natural feed; the body weight of larvae and pupas of the daphnis nerii, which is fed with the feed, at each age period is relatively heavy; the development period of the larvae is relatively short and the survival rate is relatively high.

Description

technical field [0001] The present invention relates to an insecticide and a preparation method of the insecticide, and also relates to an artificial feed and the use of the feed, in particular to a oleander hawkmoth virus insecticide and a preparation method thereof, Artificial feed for oleander hornworm and the use of the feed. Background technique [0002] Oleander hawkmoth (Daphnis nerii (Linnaeus)), also known as pink and green hawkmoth, eagle pattern hawkmoth, oleander hawkmoth, belongs to Lepidoptera (Lepidoptera), Sphingidae (Sphingidae), It is a worldwide pest and is distributed in Guangdong, Guangxi, Taiwan, Fujian, Jiangxi, Yunnan, Hunan and Sichuan provinces in China. The oleander hornworm mainly harms oleander, and also harms the medicinal plant Rauvdfiavomitoria Afzel.ExSpreng of the genus Rauvdfiasp., and other tree species in the family, as well as the soft branch yellow cicada and yellow oleander , periwinkle and other plants of the Apocynaceae family. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N47/34A01P7/04A23K10/30A23K20/174A23K20/147A23K20/105A01K67/033
CPCA01K67/033A01N47/34A01N63/10A23K10/30A23K20/105A23K20/147A23K20/174
Inventor 靳亮刘卓荣占智高王金昌关丽梅黄朝
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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