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Method for determining genome size of nymphaeaceae plants

A plant genome and water lily technology, applied in the field of plant molecular biology, can solve the problems of less research and evaluation, plant homonymous or synonymous, unclear characteristics and background, etc.

Inactive Publication Date: 2017-12-08
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

Most of the imported Nymphaea germplasm is a variety formed by hybridization of multiple sources, and the characteristics and background are not clear. The phenomenon of homonyms or different names of plants is quite common, which affects the development of commercial development and self-selected varieties.
At present, most studies focus on the relationship between some Nymphaeaceae plants, and there are few studies and evaluations on the design of Nymphaea germplasm resources. The research on Nymphaea germplasm resources based on morphological traits has not been reported, and there is no genome size suitable for Nymphaeaceae plants. The report of the measurement method

Method used

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  • Method for determining genome size of nymphaeaceae plants
  • Method for determining genome size of nymphaeaceae plants
  • Method for determining genome size of nymphaeaceae plants

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Experimental program
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Embodiment 1

[0027] 1. Compare and select the most suitable dissociation solution for Nymphaeaceae plants.

[0028] 1) Material preparation: select fresh young leaves of white water lily (Nymphaea alba L.);

[0029] 2) Selection of cell nucleus extraction buffer and preparation of cell nucleus suspension: wash the young leaves of Nymphaea alba L. with deionized water, blot the surface moisture with filter paper, weigh 20 mg and place it in a pre-cooled glass petri dish , add 1mL nuclear extraction buffer (LB01, OTTO'S, Galbraith's, GPB, Tris-MgCl 2 , WPB, mGb), then chopped the young leaves with a Geely single-sided blade, filtered through a filter with a pore size of 50 μm to obtain a cell nucleus suspension, and placed it on ice for future use. The formulas of the seven nuclear extraction buffers are: 1. Galbraith's buffer: 45mM MgCl 2 , 20mM MOPS, 30mM sodium citrate, 0.1% (vol / vol) Triton X-100. Adjust the pH to 7.0 with 1M sodium hydroxide, filter through a 0.22 μm filter membrane,...

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Abstract

The invention provides a method for determining the genome size of nymphaeaceae plants. The method is characterized by determining the genome size of three nymphaeaceae plants of NymphaeamexicanaZucc, NymphaeastellataWilld and Nymphaea alba L. by using Oryzasativa nipponbare and Zea mays L B73 as interior labels and utilizing flow cytometry. A result shows that the three plants are different in genome size, wherein the genome size of the Nymphaea alba L. is the largest, and the genome size of the NymphaeamexicanaZucc is the smallest. Basic data reference is provided for genomics and population evolution research of the plants.

Description

Technical field: [0001] The invention belongs to the field of plant molecular biology. It specifically relates to a method for measuring the genome size of Nymphaeaceae plants using flow cytometry. Background technique: [0002] The total amount of DNA contained in the haploid genome of an organism is called the C value, which represents the size of the genome of the organism and is an important feature of the organism. Since the 1950s, people have gradually realized that there will be great differences in the amount of nuclear DNA among different organisms, and tried to explain this phenomenon. The most famous one is the "C-value paradox" theory, that is, the genome There is no correlation between size and the complexity of an organism. C-value is the basic data for judging the genome size of a species, mastering these data will be beneficial to the formulation of genetics and genomics research programs and data analysis. [0003] Flow cytometry (FCM) is a technology tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N1/28
CPCG01N1/28G01N1/286G01N15/1434
Inventor 杨俊波贾艳霞贺正山
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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