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A seabuckthorn leaf polypeptide and its preparation method

A technology of seabuckthorn leaf and enzymatic hydrolysis, which is applied in the field of food processing, can solve the problems such as no report on the preparation method of seabuckthorn leaf polypeptide, achieve good market application prospects, produce no toxic substances, and low energy consumption

Active Publication Date: 2021-03-19
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report on the preparation method of seabuckthorn leaf polypeptide

Method used

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  • A seabuckthorn leaf polypeptide and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 preparation method of seabuckthorn leaf polypeptide of the present invention

[0025] (1) Pretreatment: crush seabuckthorn leaves, mix with boric acid buffer according to the ratio of 1g:50mL of material to liquid, adjust the pH value to 10, put it in a constant temperature water bath at 90°C to inactivate the enzyme for 10min, cool to 45°C, Get the pretreatment solution;

[0026] (2) Enzymolysis: Add alkaline protease to the pretreatment solution, the enzyme addition amount is 14000U / g, put it in a constant temperature water bath at 45°C, and enzymolyze it for 2 hours. After the reaction is completed, put it in a water bath at 90°C to kill the enzyme. Cool to room temperature to obtain the enzymatic hydrolysis solution;

[0027] (3) Clarification: centrifuge the enzymolysis solution at 5000r / min for 10min, take the supernatant in a container, and obtain the polypeptide solution.

Embodiment 2

[0028] Embodiment 2 preparation method of seabuckthorn leaf polypeptide of the present invention

[0029] (1) Pretreatment: crush seabuckthorn leaves, mix with boric acid buffer according to the material-liquid ratio of 1g:30mL, adjust the pH value to 8, put it in a constant temperature water bath at 70°C to inactivate the enzyme for 10min, cool to 55°C, Get the pretreatment solution;

[0030] (2) Enzymolysis: Add alkaline protease to the pretreatment solution, the enzyme addition amount is 10000U / g, put it in a constant temperature water bath at 40°C, and enzymolyze it for 4 hours. After the reaction is completed, put it in a water bath at 90°C to kill the enzyme. Cool to room temperature to obtain the enzymatic hydrolysis solution;

[0031] (3) Clarification: centrifuge the enzymolysis solution at 5000r / min for 10min, take the supernatant in a container, and obtain the polypeptide solution.

Embodiment 3

[0032] Embodiment 3 preparation method of seabuckthorn leaf polypeptide of the present invention

[0033] (1) Pretreatment: Pulverize seabuckthorn leaves, mix with boric acid buffer according to the ratio of 1g:60mL of material to liquid, adjust the pH value to 12, put it in a constant temperature water bath at 90°C for 10 minutes, and cool to room temperature to obtain pretreatment liquid;

[0034] (2) Enzymolysis: Add alkaline protease to the pretreatment solution, the enzyme addition amount is 18000U / g, put it in a constant temperature water bath at 55°C, and enzymolyze it for 2 hours. After the reaction is completed, put it in a water bath at 100°C to kill the enzyme. Cool to room temperature to obtain the enzymatic hydrolysis solution;

[0035] (3) Clarification: centrifuge the enzymolysis solution at 5000r / min for 10min, take the supernatant in a container, and obtain the polypeptide solution.

[0036] Prove beneficial effect of the present invention by concrete experi...

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Abstract

The invention discloses hippophae rhamnoides leaf polypeptide and a preparation method thereof. The preparation method comprises the steps as follows: pretreatment: hippophae rhamnoides leaves are crushed and uniformly mixed with a buffer solution in the solid-liquid ratio being 1 g:(30-60) mL, the pH value is adjusted to 8.0-12.0, the enzyme activity is passivated through thermal treatment, cooling is performed, and a pretreated solution is obtained; (2) enzymolysis: protease is added to the pretreated solution, an enzymolysis reaction is performed, the enzyme activity is passivated through thermal treatment after the reaction ends, the product is cooled to the room temperature, and an enzymatic hydrolysate is obtained; (3) clarification: the enzymatic hydrolysate is subjected to centrifugal separation, a supernatant is taken, and a polypeptide solution is obtained. The method has high protein hydrolysis degree and high polypeptide yield, has the advantages that the cost is low, the operation is simple, the condition is mild, energy consumption is low, nutrition is not damaged, toxic substances are not produced and the like, can be used for effectively preparing the hippophae rhamnoides leaf polypeptide and has bright market application prospects.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method of seabuckthorn leaf polypeptide. Background technique [0002] Seabuckthorn (Hippophaer hamnoides L.) is a shrub or small tree of the genus Acanthopanax in the family Elaeagnaceae. Its fruit is a drug listed in the Pharmacopoeia of the People's Republic of China, and it is a resource for both medicine and food recognized by the state. Therefore, the research and utilization of the fruit are paid more and more attention to, and there are more and more beverages, food, health care products and medicines processed with the fruit. However, the utilization of seabuckthorn leaves is not much at present, mainly limited to the extraction of tea and flavonoids. [0003] According to literature reports, seabuckthorn leaves, like seabuckthorn fruits, contain extremely rich nutrients and physiologically active substances. Seabuckthorn leaves are rich in amino a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 左蕾蕾焦婷邹丽霞孟晓
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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