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A kind of bacillus licheniformis producing tetramethylpyrazine and its application

A technology of Bacillus licheniformis and tetramethylpyrazine, which is applied in the field of fermentation engineering, can solve problems such as the inability to meet industrial production functions, and achieve the effects of being conducive to cell growth, transformation and upgrading, and ecological manufacturing, and improving the health and active substances of liquor.

Active Publication Date: 2019-11-15
ANHUI RUISIWEIER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Previously, the inventors used four strains of Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, and Bacillus lipolytica to carry out experiments on producing tetramethylpyrazine in single and mixed strain culture modes respectively. The results showed that although these strains were tested The yield of indoor tetramethylpyrazine is relatively high, but in the high-temperature Daqu culture mode, the content of tetramethylpyrazine is only 1 / 10 to 1 / 5 of that in the medium-temperature shelf Daqu culture mode, which is far from meeting the industrial production function of Daqu needs

Method used

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  • A kind of bacillus licheniformis producing tetramethylpyrazine and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation of Bacillus licheniformis functional bacteria culture fluid

[0033] Use an inoculation loop to pick 1-2 rings of Bacillus licheniformis GJBL055 strains stored on the slope in 300-400mL liquid medium, and cultivate them on a shaker at 120rpm at 37°C for 24h to obtain the first-grade seed liquid; Inoculate the inoculum of 10% of the inoculum into the fermenter, cultivate at 80 rpm at 37°C for 24 hours to obtain the second-grade seed liquid; then inoculate the second-grade seed liquid at 10% inoculum in the fermenter, and cultivate at 80 rpm at 37°C for 24 hours to obtain the third-grade seed liquid. The seed solution is the functional bacteria culture solution;

[0034] The 10% inoculation amount refers to the ratio of the volume of the primary or secondary seed solution to the volume of the fermenter.

[0035] Described liquid culture medium constitutes as follows by mass concentration:

[0036] Glucose 1wt%, yeast extract 1wt% (purchased...

Embodiment 2

[0037] Embodiment 2: the optimization of the addition amount of Bacillus licheniformis functional bacteria culture solution

[0038] Add the functional bacteria culture solution obtained in Example 1 to the water storage tank at the rate of 0.1%, 0.5%, and 1% of the koji mass per room, and after mixing, add the koji-making material whole wheat flour, mix evenly, and press the shelf-style Daqu cultivation method.

[0039] When the different addition amount of table 1, tetramethylpyrazine output

[0040] Addition amount of functional bacteria solution Content of tetramethylpyrazine in atrial flexor in ppm 0.1％ 106.3 0.5％ 389.4 1％ 356.4

[0041] It can be seen from Table 1 that when the addition ratio of Bacillus licheniformis functional bacteria culture solution is 0.5%, the content of tetramethylpyrazine in Atrial Quercus is relatively high.

Embodiment 3

[0042] Example 3: Arrangement of functional frame Daqu

[0043] Add the functional bacteria culture solution obtained in Example 1 to the water storage tank at a rate of 0.5% of the koji mass per room, add the koji-making material whole wheat flour after mixing, fully mix the material by stepping on it, and control the moisture content to 36- 37%, to obtain bent billets, the thickness of each bent billet is controlled at 6.9-7.1cm; put the bent billets in a straight line on the curved frame, and place 32-34 curved billets on each shelf, and the distance between each curved frame 5-8 cm, there are three placement schemes in total, namely "five-layer shelf with six-layer curved", "five-layer shelf with five-layer curved (the bottom floor is not placed)", "four-layer shelf with five-layer curved", a total of Place 3 rows and 10 columns of curved racks.

[0044] Table 2 The content of tetramethylpyrazine in the atrial song when different arrangements of Daqu are arranged in the f...

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Abstract

The invention discloses a tetramethylpyrazine-producing Bacillus licheniformis and its application, wherein the classification of the Bacillus licheniformis is named Bacillus licheniformis GJBL055 (Bacillus licheniformis GJBL055), which has been preserved in the China Type Culture Collection Center, The date of deposit is April 24, 2017, the deposit number is CCTCC NO: M 2017204, and the deposit address is Wuhan University, Wuhan, China. Using the medium-temperature shelf culture mode, the Bacillus licheniformis of the present invention is inserted into the plate Daqu, and through the pre-cultivation of functional microorganisms, the construction of the space model of the shelf song, and the four-stage regulation of the room temperature, a high-yielding medium-temperature condition is formed. The Daqu manufacturing process of tetramethylpyrazine increases the content of tetramethylpyrazine in Daqu to 400mg / kg.

Description

technical field [0001] The invention relates to a tetramethylpyrazine-producing bacillus licheniformis and an application thereof, belonging to the technical field of fermentation engineering. Background technique [0002] "Qu is the bone of wine", and Daqu plays a vital role in the production of liquor. The purpose of the preparation is: the formation of multi-functional bacterial strain, multi-enzyme system and various aroma-generating substances. In the Daqu production process, bacteria and yeasts are first fermented to increase the temperature of the koji core, and the heat is dissipated to form a surface layer suitable for mold growth; then, with the dispersal of water, mold grows inside, and the curved surface gradually dries to form a closed layer. The biological heat generated by the multi-bacteria fermentation in Daqu is blocked, forming a higher "top fire temperature", and the aroma components are also produced at this time. In order to achieve the above goals, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12G3/02C12R1/10
CPCC12G3/02C12N1/205C12R2001/10
Inventor 葛向阳何宏魁李晓欢张严
Owner ANHUI RUISIWEIER TECH
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