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A method for separating and measuring different polarity and molecular weight components of humic acid

A humic acid and molecular weight technology, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of affecting function performance, complicated procedures, time-consuming and sample consumption, etc., and achieves high sensitivity, convenient operation, and stable testing.

Active Publication Date: 2020-05-22
TSINGHUA UNIV
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Problems solved by technology

[0004] In addition to polarity, the molecular weight of humic acid also has an important impact on its function. In media such as soil, sediment, and water, there are differences in the contact area and probability of humic acid with different molecular weights and minerals and microorganisms, which affects its function.
However, under normal circumstances, there is no significant correlation between the polarity and molecular weight of humic acid. Most of the current determination methods only measure the polarity or molecular weight of humic acid, which is time-consuming and sample-consuming. Simultaneous determination of polarity and molecular weight characteristics will effectively promote the analytical research on the structure and composition of humic acid

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  • A method for separating and measuring different polarity and molecular weight components of humic acid
  • A method for separating and measuring different polarity and molecular weight components of humic acid

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Embodiment Construction

[0025] The implementation of the present invention will be described in detail below in conjunction with the drawings and examples.

[0026] A kind of separation of the present invention and the method for measuring humic acid different polarities and molecular weight components, main steps are:

[0027] 1) Humic acid sample pretreatment: Dissolve humic acid powder in phosphate buffer solution, pass through a 0.45 μm filter membrane after the humic acid is fully dissolved, and measure the dissolved organic carbon (DOC) content of the humic acid solution.

[0028] 2) Adjust the DOC of the humic acid solution with 0.2M phosphate buffer (pH=7), pipette 1mL of the solution into a brown liquid phase sample bottle, put it into a sample tray, and put it on the machine for testing.

[0029] 3) High-performance liquid chromatography module construction: the module includes mobile phase, binary pump, six-way valve, sample injector, column thermostat, DAD detector and FLD detector, and e...

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Abstract

A method for separating and measuring humic acid components with different polarities and molecular weights comprises the following steps: 1) pretreatment of a humic acid sample, 2) setting up of a high performance liquid chromatography module, 3) setting of determination parameters, and 4) determination of the sample. The method has the advantages of convenient operation, stable test, effective separation of the water-soluble humic acid components with the different polarities and molecular weights, and is of great significance for exploring the composition of humic acid.

Description

technical field [0001] The invention belongs to the technical field of organic matter structure characterization, in particular to a method for separating and measuring components of different polarities and molecular weights of humic acid. Background technique [0002] Humic acid widely exists in the environment, such as soil, sediment, surface, groundwater, etc., and their composition structure is complex, including a variety of components. There are differences in polarity and molecular weight of these humic acid components, but due to the tight combination of different components, it is difficult to identify components with different polarity and molecular weight by conventional spectral means, giving humic acid composition structure identification bring inconvenience. [0003] The polarity of humic acid will affect its function in aqueous solution. Relatively hydrophilic humic acid components are more likely to perform their functions in aqueous solution, which in turn...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14G01N2030/146
Inventor 张芳袁英李广贺张旭侯德义
Owner TSINGHUA UNIV
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