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Structured biological samples for analysis by mass cytometry

A biologically sampled, structured technology for application-specific bioreactors/fermenters, analytical materials, biomass post-processing, etc., which can solve problems such as limiting flushing time

Pending Publication Date: 2017-12-01
FLUIDIGM CANADA INC
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  • Abstract
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Problems solved by technology

Furthermore, the gas dynamic diffusion of the aerosol plume during transport to the ICP source further limits the washout time

Method used

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  • Structured biological samples for analysis by mass cytometry
  • Structured biological samples for analysis by mass cytometry
  • Structured biological samples for analysis by mass cytometry

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Embodiment Construction

[0030] Structured biological materials are used to introduce discrete samples or pixels of biological material into the ionization source of a mass cytometer or mass spectrometer, such as an ICP source. Structured biomaterials refer to materials arranged in discrete addressable sites on a support, such as individual cells, tissue parts, or other aggregates of biological material. The structured biomaterial can be released from the carrier and each discrete pixel of material introduced sequentially into a mass cytometer or mass spectrometer for analysis. In contrast to random introduction of biological samples as in conventional mass cytometry or mass spectrometry, advantages of sequential introduction of discrete material pixels include higher sample processing rates and elimination of multimers caused by simultaneous arrival of multiple entities, Such as dimers, trimers, etc. Handling and analysis of data can also be simplified by eliminating multimers.

[0031] "Pixel" ref...

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Abstract

Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to and benefit of U.S. Provisional Patent Application 62 / 108,911, filed January 28, 2015, and U.S. Provisional Patent Application 62 / 098,890, filed December 31, 2014, the contents of which are incorporated herein in their entirety. field of invention [0003] The present disclosure relates to devices and methods for introducing a biological sample into a mass cytometer. Background of the invention [0004] Mass cytometry is a newly developed technique for the study of biological samples. The technique was originally developed for the study of cell populations, where a sample of interest containing cells of various organisms is "stained" with affinity probes, such as antibodies, attached to elemental tags. The amount of a given type of affinity probe attached to each cell can be used to characterize each individual cell. The amount of each type of affinity probe is directly related to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34G01N35/10H01J49/04
CPCG01N2001/045H01J49/04G01N33/4833G01N2001/2886H01J49/0027
Inventor A.V.洛博达
Owner FLUIDIGM CANADA INC
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